Protein Info for BPHYT_RS25160 in Burkholderia phytofirmans PsJN

Annotation: NAD/NADP-dependent betaine aldehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01804: betaine-aldehyde dehydrogenase
  • amino acids 10 to 476 (467 residues), 733 bits, E=5.9e-225
• PF00171: Aldedh
  • amino acids 15 to 478 (464 residues), 603.6 bits, E=1.1e-185

Best Hits

Swiss-Prot: 100% identical to BETB_PARPJ: NAD/NADP-dependent betaine aldehyde dehydrogenase (betB) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K00130, betaine-aldehyde dehydrogenase [EC: 1.2.1.8] (inferred from 100% identity to bpy:Bphyt_5059)

MetaCyc: 70% identical to betaine aldehyde dehydrogenase (Escherichia coli K-12 substr. MG1655)
Betaine-aldehyde dehydrogenase. [EC: 1.2.1.8]

Predicted SEED Role

"Betaine aldehyde dehydrogenase (EC 1.2.1.8)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.2.1.8)

MetaCyc Pathways

• choline-O-sulfate degradation (3/3 steps found)
• glycine betaine biosynthesis III (plants) (3/3 steps found)
• choline degradation I (2/2 steps found)
• glycine betaine biosynthesis I (Gram-negative bacteria) (2/2 steps found)
• glycine betaine biosynthesis II (Gram-positive bacteria) (2/2 steps found)
• β-alanine biosynthesis I (1/2 steps found)
• β-alanine biosynthesis IV (1/2 steps found)
• choline degradation IV (2/4 steps found)
• dimethylsulfoniopropanoate biosynthesis I (Wollastonia) (1/3 steps found)
• dimethylsulfoniopropanoate biosynthesis II (Spartina) (1/4 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.8

Use Curated BLAST to search for 1.2.1.8

Sequence Analysis Tools

See B2TCJ9 at UniProt or InterPro

Protein Sequence (489 amino acids)

>BPHYT_RS25160 NAD/NADP-dependent betaine aldehyde dehydrogenase (Burkholderia phytofirmans PsJN)
MAVFATQRLYIGGGYVDATSGETFDTLDPATGETLASVQQASAADVDRAVRSAKQGQREW
AALTAMQRSRILRRAVDLLRERNDELAALETRDTGKPIAETLAVDIVTGADVIEYYAGLA
TAIEGQQIPLRPTSFVYTRREPLGVCAGIGAWNYPIQIACWKSAPALAAGNAMIFKPSEI
TPLSALKLAEIFTEAGVPAGVFNVVQGDGRVGAMLAAHPDIEKISFTGGVETGKKVMSMA
GASSLKEVTMELGGKSPLLVFDDANLERAADIATSANFFSSGQVCTNGTRVFVQRGVLDR
FEALVLERVKRIRVGKPTDAATNFGPLVSAAQLHKVLGYIESGKQEGARLVAGGKRLTEG
HFAGGQYVEPTVFADCRDDMRIVREEIFGPVMSILVFDDEDEAIARANHTAYGLAAGVVT
ENLARAHRVIHRLEAGICWINTWGESPAEMPVGGYKQSGVGRENGITTLEHYTRIKSVQV
ELGPYQPVF