Protein Info for BPHYT_RS25025 in Burkholderia phytofirmans PsJN

Annotation: acyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 601 PF12418: AcylCoA_DH_N" amino acids 6 to 35 (30 residues), 30.5 bits, see alignment (E = 7.5e-11) PF02771: Acyl-CoA_dh_N" amino acids 47 to 157 (111 residues), 36.9 bits, see alignment E=1.1e-12 PF02770: Acyl-CoA_dh_M" amino acids 163 to 270 (108 residues), 41.8 bits, see alignment E=2.4e-14 PF00441: Acyl-CoA_dh_1" amino acids 286 to 451 (166 residues), 75.6 bits, see alignment E=1.2e-24 PF12806: Acyl-CoA_dh_C" amino acids 470 to 596 (127 residues), 104.6 bits, see alignment E=1e-33

Best Hits

KEGG orthology group: K00248, butyryl-CoA dehydrogenase [EC: 1.3.8.1] (inferred from 100% identity to bpy:Bphyt_5032)

Predicted SEED Role

No annotation

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.1

Use Curated BLAST to search for 1.3.8.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TCH2 at UniProt or InterPro

Protein Sequence (601 amino acids)

>BPHYT_RS25025 acyl-CoA dehydrogenase (Burkholderia phytofirmans PsJN)
MQDAQIINSRDLEFQLFEVLEVETLTRRARHADHSRETFDAALDTAHTVAVEKFATHNRL
ADEHEPQFDGQRVTLPAEVKDALDAMRASGFLAAGKDYEWGGMQLPSVISFACLSLFKSA
NIATASYAMLTTANANVIERFGSETQKRKYLQALFEGRAFGTMALTEPQAGSSLSDLVTS
ATPNADGTYSIRGNKIFISGGDHELSENIVHLVLARIPGGPPGVKGISLFTVPKFHVNED
GSLGARNDVALAGLIHKMGWRGTTSTMLSFGEHGECIGELVGEPHHGLAYMFHMMNEARI
GVGLGAVMLGYRGYLASLDYARERPQGRRPDNKNPLDPQLPLIEHADVRRMLLAQKAYVE
GAYALCLYAARLVDEQNTGESETVRAEAGLLLDLLTPVVKSWPSQWCLEANSLAIQIHGG
YGYTREYPVEQFYRDNRLNMIHEGTHGIQAIDLLGRKVTMKQGAALKQLAREIQQTIGTA
REHPALHAHADALGAAWTDLTATVEALLPTLTHESERALANANAFLEAFGHIVIAWTWLR
QAIVANAALSASHSEADADFYRGKLHACQWFFRWELPRVALMLATLRTLDDTTLAMAPQW
F