Protein Info for BPHYT_RS24975 in Burkholderia phytofirmans PsJN

Annotation: RNA polymerase sigma24 factor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 186 TIGR02937: RNA polymerase sigma factor, sigma-70 family" amino acids 21 to 176 (156 residues), 110.6 bits, see alignment E=3.1e-36 PF04542: Sigma70_r2" amino acids 25 to 92 (68 residues), 71.6 bits, see alignment E=5.5e-24 PF08281: Sigma70_r4_2" amino acids 120 to 172 (53 residues), 52 bits, see alignment E=6.8e-18 PF04545: Sigma70_r4" amino acids 125 to 174 (50 residues), 30.9 bits, see alignment E=2.3e-11

Best Hits

KEGG orthology group: K03088, RNA polymerase sigma-70 factor, ECF subfamily (inferred from 100% identity to bpy:Bphyt_5021)

Predicted SEED Role

"RNA polymerase sigma-70 factor" in subsystem Transcription initiation, bacterial sigma factors

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TCG1 at UniProt or InterPro

Protein Sequence (186 amino acids)

>BPHYT_RS24975 RNA polymerase sigma24 factor (Burkholderia phytofirmans PsJN)
MSERDPDAQLLEQIGRQDPAAVRSLVARKLPRLLALATRMLGDRMEAEDVAQEVFVRIWK
QASRWREGEAKFDTWIHRVALNLCYDRLRGRREEPRDELPEEADPAPLPEAGLEARAQDQ
RVRDALAALPARQREALVLNYYQEMSNIDAAALMGISVDALESLLARARRNLRAQLAGSG
LSKDKS