Protein Info for BPHYT_RS24925 in Burkholderia phytofirmans PsJN

Annotation: cytochrome O ubiquinol oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 201 transmembrane" amino acids 24 to 46 (23 residues), see Phobius details amino acids 64 to 85 (22 residues), see Phobius details amino acids 93 to 111 (19 residues), see Phobius details amino acids 131 to 158 (28 residues), see Phobius details amino acids 177 to 198 (22 residues), see Phobius details PF00510: COX3" amino acids 17 to 197 (181 residues), 51 bits, see alignment E=9.9e-18 TIGR02842: cytochrome o ubiquinol oxidase, subunit III" amino acids 22 to 201 (180 residues), 266.3 bits, see alignment E=9.3e-84

Best Hits

Swiss-Prot: 63% identical to CYOC_PSEAE: Cytochrome bo(3) ubiquinol oxidase subunit 3 (cyoC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02299, cytochrome o ubiquinol oxidase subunit III [EC: 1.10.3.-] (inferred from 100% identity to bpy:Bphyt_5011)

MetaCyc: 60% identical to cytochrome bo terminal oxidase subunit III (Pseudomonas putida KT2440)
RXN0-5268 [EC: 7.1.1.3]

Predicted SEED Role

"Cytochrome O ubiquinol oxidase subunit III (EC 1.10.3.-)" in subsystem Terminal cytochrome O ubiquinol oxidase or Terminal cytochrome oxidases (EC 1.10.3.-)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.10.3.-

Use Curated BLAST to search for 1.10.3.- or 7.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TCF1 at UniProt or InterPro

Protein Sequence (201 amino acids)

>BPHYT_RS24925 cytochrome O ubiquinol oxidase (Burkholderia phytofirmans PsJN)
MLTKTMDAHSHHDADHVPSQSVFGFWLYLMTDCIIFAALFAVFAVMGHQFAGGPSGKDLF
EIPGVALETTMLLLSSITYGFAMLGAHKNRKGVLLFWLGVTFLLGLAFLVLELREFSHLI
AEGAGPSRSAFLSSFFTLVGTHGLHVTCGLIWMVVLAIQVISHRDLTERDMIRLTCLSLF
WHFLDVVWIGVFTFVYLASVI