Protein Info for BPHYT_RS24820 in Burkholderia phytofirmans PsJN

Annotation: cobyrinic acid a,c-diamide synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01656: CbiA
  • amino acids 8 to 175 (168 residues), 46.9 bits, E=3.9e-16
• PF07685: GATase_3
  • amino acids 237 to 418 (182 residues), 120.1 bits, E=1.4e-38

Best Hits

Swiss-Prot: 63% identical to COBB_PSEAE: Hydrogenobyrinate a,c-diamide synthase (cobB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02224, cobyrinic acid a,c-diamide synthase [EC: 6.3.1.- 6.3.5.9] (inferred from 100% identity to bpy:Bphyt_4996)

Predicted SEED Role

"Cobyrinic acid A,C-diamide synthase" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis

MetaCyc Pathways

• adenosylcobalamin biosynthesis II (aerobic) (30/33 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (30/36 steps found)
• cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) (14/15 steps found)
• cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) (12/13 steps found)

KEGG Metabolic Maps

• Porphyrin and chlorophyll metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.- or 6.3.5.9

Sequence Analysis Tools

See B2TCD6 at UniProt or InterPro

Protein Sequence (432 amino acids)

>BPHYT_RS24820 cobyrinic acid a,c-diamide synthase (Burkholderia phytofirmans PsJN)
MAACPALFISAPASGQGKTTITAGLARYHRRLGRRVRVFKTGPDFLDPMILARASGAPVL
SLDLWMVGEAACRTLLAQAAAEADLILIEGVMGLFDGTPSSADLATTFGVPVLAVISAKA
MAQTFGAVAFGLAQFRPQVPFYGVLANRVGSARHAQMLEEALSPALRWCGHIAASDEITL
PDRHLGLHQADEIDDLDARLDHAADTLGRTALAELPPPVEFGAPTPEVLPRLLDGKTIAV
ARDAAFSFIYPANLALLEVLGAQVAFFSPLADEALPEHADAVYLPGGYPELHAAALANNN
RSAASIHAHAAAGKPLVAECGGMLYLLDQLTDTHGVTTPMLGLLPGHATMQTRFTALGMQ
QIDSLHGPMTGHTFHYSKLNTSLTPLRSATRPQGDAPGEAVYRAGSIVATYMHAYWPSNP
AFTAALFHGEAF