Protein Info for BPHYT_RS24765 in Burkholderia phytofirmans PsJN

Annotation: FAD-dependent oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 831 PF01266: DAO" amino acids 10 to 380 (371 residues), 252.5 bits, see alignment E=2.3e-78 PF16350: FAO_M" amino acids 384 to 438 (55 residues), 73.2 bits, see alignment 3.9e-24 PF01571: GCV_T" amino acids 440 to 712 (273 residues), 272.6 bits, see alignment E=7.3e-85 PF08669: GCV_T_C" amino acids 737 to 823 (87 residues), 62 bits, see alignment E=1e-20

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_4985)

Predicted SEED Role

"Sarcosine dehydrogenase (EC 1.5.99.1)" (EC 1.5.99.1)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TCC5 at UniProt or InterPro

Protein Sequence (831 amino acids)

>BPHYT_RS24765 FAD-dependent oxidoreductase (Burkholderia phytofirmans PsJN)
MSTPLPTHARVVIVGGGIIGCSVAYHLTKLGWTDVVLLEQGQLSCGTTWHAAGLVGQLRA
QESMTKLIRYSTALYAELEADTGLATGWKQCGSLSVARTAERMTQLKRTAAVARAYGVAC
DVISPREAGDLWPVMRTDDLLGAVWLPGDGKANPTDLTQALARGARQRGARIVENTRVTA
IHTRTAQDGKANGAREVSGLAWRNKDGEEGTLGADIVVNCAGQWAKGVGRLCGVTVPLHS
AEHYYIVTERIAGVHPDLPVMRDPDGFIYFKEEVGGLVMGGFEPDAKPWGMNGIPENFEF
QLLPDDWDQFEILMKNALQRVPALETAQVKQFYNGPESFTPDNNFMLGEAPELRRFFVGA
GFNSMGIASAGGAGMALAEWIVAGEPTMDLWPVDIRRFARFNGNDTWLHDRVKETLGLHY
AMPWPNRELDSARPFRRSPLYSLLRDEGACFGSKMGWERANFFAPSPDEARIEYAFGQQN
WLPWSGAEHRACREGVALFDMTSFSKFLVKGRDAQSVLQGLVANDVDVPNGTTVYTAMLN
ERGGYESDFTLTRLADDQYLLVTGTAQTTRDFDSIEKSIPHDRHCTLVDVTGQYAVLAVM
GPRSRELLQSVSKADWSNEAFAFGQSRELDLGYATVRATRLTYVGELGWELYVPVEFAVG
VYETLHAAGKAFGLVNAGYYAIDSLRIEKGYRAWGRELTPDTNPFEAGLSFACKLDKDIA
FRGRDALLKLRAEPLRRRMVVLSANGATDRMLWGGEAILRDGKPVGFVSSAAFGHTLGCP
VAMGYVNNPDGAADAEYLNSGSYAIDVAGELLPATLHLKAPYDPRSERVKC