Protein Info for BPHYT_RS23550 in Burkholderia phytofirmans PsJN

Annotation: multidrug transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1041 signal peptide" amino acids 7 to 11 (5 residues), see Phobius details transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 334 to 353 (20 residues), see Phobius details amino acids 360 to 380 (21 residues), see Phobius details amino acids 387 to 411 (25 residues), see Phobius details amino acids 431 to 453 (23 residues), see Phobius details amino acids 463 to 490 (28 residues), see Phobius details amino acids 528 to 548 (21 residues), see Phobius details amino acids 860 to 878 (19 residues), see Phobius details amino acids 885 to 905 (21 residues), see Phobius details amino acids 911 to 935 (25 residues), see Phobius details amino acids 957 to 977 (21 residues), see Phobius details amino acids 989 to 1015 (27 residues), see Phobius details PF00873: ACR_tran" amino acids 3 to 1015 (1013 residues), 1041.5 bits, see alignment E=0 PF03176: MMPL" amino acids 288 to 499 (212 residues), 30.1 bits, see alignment E=2.5e-11

Best Hits

Swiss-Prot: 59% identical to MDTB_ECOL5: Multidrug resistance protein MdtB (mdtB) from Escherichia coli O6:K15:H31 (strain 536 / UPEC)

KEGG orthology group: K07788, RND superfamily, multidrug transport protein MdtB (inferred from 66% identity to bmu:Bmul_2105)

MetaCyc: 59% identical to multidrug efflux pump RND permease subunit MdtB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-352; TRANS-RXN-353; TRANS-RXN-92

Predicted SEED Role

"Cobalt-zinc-cadmium resistance protein CzcA; Cation efflux system protein CusA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TDR7 at UniProt or InterPro

Protein Sequence (1041 amino acids)

>BPHYT_RS23550 multidrug transporter (Burkholderia phytofirmans PsJN)
MNFSRLFILRPVATSLLMVALVLVGLVAVRFLPVSSLPDVDYPTIQVQTFYPGASPNVMA
TTVTAPLEVQLGEIPGLQQMVSYSSEGASVITLQFDLSLNLDIAEQNVQQAINAANSFLP
AGLPAPPTYAKVNPADQPILTLAVTSKSMSLTQLQDTANNRLATKISEVPGVGLVTTAGG
NVPAVRVEADPQKLAAYGLNLDDLRTLLANVNVSQPKGNFDGPELDYTINANDQIVDPQD
YLATVIAYQNGAPVYLRDVARVSQAAQDVERGAWYNRTPAIVLNVQRQPGANVIATVNQI
MKQLPALESTLPAGLQVTVVANTTGVIRSSVSDAAFELVLAVVLVVLVIFVFLRNVPATI
IPSISVPVSLIGALAVMYQLNYSIDNLSLMALIIATGFVVDDSIVMIENIVRYLEEGKTP
LEAALEGAGQIGFTILSLTVSLIAVLIPLLFMGGVIGRLFSEFAITLAVTIVISAVVSLT
VVPMLCARLLRAQAQRHPSRFERISEGLFDKTLAAYERGLVWVLDHQLLTLVVALITVAL
TAILYIVIPKGLFPVQDVGVIQGISVADNSVSYTAMVRRQSALADAILKDPDVVSLTSYV
GIDGTNPTLNNGRFLINLRPRDDRSITAEEIGRRIQQEVADVPGIRLYLQPEQDLTLDTS
ISRNQYNFVLRGPNQQAFNQYVPALIARMKQISAITDVTSDLNTNGLSVNVEVNRQLAAR
YGITAATIDNALYDALGQRIVSTIFQQSDQYRVILVAKPETIPTVQSIGNLYLPSQTSST
GQVPLSGIAKISITQSPLLISHLAQFPSVTISFNLAQDASLSAAVAQIRDAEKAVALPPS
ITTSFQGAAQAFEDSLSSEVYLLIAALVAVYIVLGVLYESYIHPVTILSTLPSAGIGALL
ALMIAGSDLDVIGIIGIVLLIGIVKKNAIMMVDFALEAERVHGKPPREAIFEASLLRFRP
ILMTTLAAMLGALPMLLGTGTGSELRRPLGLAIIGGLTLSQVLTLFTTPVIYLFFDRLAQ
RVKNRRRDVNDGAAAPPENAP