Protein Info for BPHYT_RS23420 in Burkholderia phytofirmans PsJN

Annotation: acetoacetyl-CoA synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1021 transmembrane" amino acids 769 to 788 (20 residues), see Phobius details TIGR01217: acetoacetate-CoA ligase" amino acids 20 to 653 (634 residues), 583.8 bits, see alignment E=1.9e-179 PF00501: AMP-binding" amino acids 117 to 440 (324 residues), 132.6 bits, see alignment E=4.2e-42 PF00550: PP-binding" amino acids 682 to 744 (63 residues), 40.2 bits, see alignment 8.4e-14 PF00975: Thioesterase" amino acids 766 to 1014 (249 residues), 86.8 bits, see alignment E=6.5e-28 PF00561: Abhydrolase_1" amino acids 767 to 872 (106 residues), 27.7 bits, see alignment E=5.5e-10 PF12697: Abhydrolase_6" amino acids 783 to 1000 (218 residues), 29.4 bits, see alignment E=3.2e-10

Best Hits

KEGG orthology group: K01907, acetoacetyl-CoA synthetase [EC: 6.2.1.16] (inferred from 100% identity to bpy:Bphyt_4720)

Predicted SEED Role

"Acetoacetyl-CoA synthetase (EC 6.2.1.16)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Polyhydroxybutyrate metabolism (EC 6.2.1.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.16

Use Curated BLAST to search for 6.2.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TDP6 at UniProt or InterPro

Protein Sequence (1021 amino acids)

>BPHYT_RS23420 acetoacetyl-CoA synthase (Burkholderia phytofirmans PsJN)
MNHENFVGASMRMYAREPVFQNLPERVAGSRMSRFTAALENFTGERFADYPELHAYSTRE
FRRFWQCFLQWTEGMEWGGKAEPACVGDECETASFFPNVELNYAQSVLGSKIAPDESPAL
TARYADGRRETMTRGELRERVARLACSLNELGLCPGDRAVAIMRNDAHAIIAALAVTALG
ATLSTAAPETGVQAILDRFEPLEPRILFAHTTQRSFDTAGSIASHVAAVAAALPTLTHVV
CLDETPLPSTVSQPQHSLRDLIVQGDAARFAWRRFPFNHPLFIMFSSGTTGKPKCIVHGA
GGTLLEHLKEHQLHSDLGPGDKLYFHTSCSWMMWNWQLSALASGVEIVTYDGPVSEVDTL
WRMVADERVTVFGTSPAYLKMCEDAGLKPGEQFGLHALRAMMSTGAVLYDSQFEWVRAYV
KPLQLQSISGGTDIIGCFVLGNPNLPVYAGEAQCRSLGLDVQAWNEGAPTSMTGELVCVN
PFPSRPLGFFGDADGSRFHAAYFKANPGVWTHGDIIEFSAQGSARLHGRSDGVLNVRGIN
VSPGEIYRIVSGIGEINQSMVVAQTTHDASGSGQRVVLLLVLRRGAKMSAALASRVRREL
MLQGSAALVPDVIAEVEALPVTHNGKASEAAARDAVNGLPVRNLSSLANPGCVEKISAHP
ALARTRRELPEPGDSAEQVEVYLCALWEQLFSFSPVSRDDNFFELGGHSLLAAQMLAEIR
LATGRTLPLATLIIAPTIARLATVITGERTQDAHPNVVPMRAGRGRPVFMLHSITGSVME
CLTLAGALASERPVYGLQARGLDGDEEPQRCVEEMARVYVRQMRAVQPRGPYALVGYSFG
GLVAFEMAQQLVAAGEKIELLCLLDTYVDERYLPLHEWLSFQYEVMAERVRAFRALSARG
RMAYMKDRVFGAADRIRMRLGRMAARPAADTQGLPPVLRQVRESMRVAMATYRPRRYLGS
PIVYVRASGREGGQGDPLPAWQRVARSGLLVKTIDGAHTDLVVEPNLAMVADTLARRLAG
A