Protein Info for BPHYT_RS23260 in Burkholderia phytofirmans PsJN

Annotation: isovaleryl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 393 PF02771: Acyl-CoA_dh_N" amino acids 13 to 124 (112 residues), 148.7 bits, see alignment E=1.7e-47 PF02770: Acyl-CoA_dh_M" amino acids 128 to 223 (96 residues), 98.1 bits, see alignment E=5.3e-32 PF00441: Acyl-CoA_dh_1" amino acids 235 to 388 (154 residues), 131 bits, see alignment E=8.4e-42 PF08028: Acyl-CoA_dh_2" amino acids 257 to 371 (115 residues), 51.6 bits, see alignment E=2.3e-17

Best Hits

Swiss-Prot: 65% identical to IVD_RAT: Isovaleryl-CoA dehydrogenase, mitochondrial (Ivd) from Rattus norvegicus

KEGG orthology group: K00253, isovaleryl-CoA dehydrogenase [EC: 1.3.99.10] (inferred from 100% identity to bpy:Bphyt_4686)

Predicted SEED Role

"Isovaleryl-CoA dehydrogenase (EC 1.3.8.4); Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.8.1)" (EC 1.3.8.1, EC 1.3.8.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.1

Use Curated BLAST to search for 1.3.8.1 or 1.3.8.4 or 1.3.99.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TDL4 at UniProt or InterPro

Protein Sequence (393 amino acids)

>BPHYT_RS23260 isovaleryl-CoA dehydrogenase (Burkholderia phytofirmans PsJN)
MSNLPGLQFPLGEEIEMLRDSIAGFAAKEIAPRAAEIDRTDQFPMDLWRKFGDLGVLGMT
VSEEYGGANMGYTAHMIAMEEISRASASVGLSYGAHSNLCVNQIHRNGTEAQKQKYLPKL
VSGEHVGALAMSEPNAGSDVVSMKLRAEKKGDHYVLNGTKMWITNGPDCDTLVVYAKTDP
EANSRGITAFIVEKGMKGFSVAQKLDKLGMRGSHTGELVFQDVEVPEENILGQLNGGAKV
LMSGLDYERAVLAGGPTGIMVAVMDAVVPYIHDRKQFGQSIGEFQLIQGKVADLYTTLQA
CRAYLYAVGRQLDTLGKEHVRQVRKDCAGVILYTAEKATWMAGEAIQILGGNGYINEYPV
GRLWRDAKLYEIGAGTSEIRRMLIGRELFAETA