Protein Info for BPHYT_RS22965 in Burkholderia phytofirmans PsJN

Annotation: ligand-gated channel protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 804 transmembrane" amino acids 32 to 58 (27 residues), see Phobius details PF07715: Plug" amino acids 93 to 202 (110 residues), 56.9 bits, see alignment E=2.7e-19 PF00593: TonB_dep_Rec_b-barrel" amino acids 343 to 780 (438 residues), 189 bits, see alignment E=2.9e-59

Best Hits

KEGG orthology group: K02014, iron complex outermembrane recepter protein (inferred from 93% identity to bpy:Bphyt_4626)

Predicted SEED Role

"Outer membrane receptor proteins, mostly Fe transport" in subsystem Hemin transport system

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TDF7 at UniProt or InterPro

Protein Sequence (804 amino acids)

>BPHYT_RS22965 ligand-gated channel protein (Burkholderia phytofirmans PsJN)
MFNFALRRLPFCACRGARGVSLQPLPRPLLSGYLPAIVPAFVPAFVPAFVPAFVPAFIAS
SAHAQSASADAALPAISVSANAAAAPRAVDPKLPASVETVTREQFDNWNVVNTEDVLKYM
PNLAVRKRFIGDLNSIIAVRGTSNTQSARGLVYADGLLLSNLLGNSYSFPPRWSMVFPDE
IQQVDVIYGPFSALYPGNSLGATVLISTRMPKQFEASADVKAFTQHFSLFGVNQNFNGSE
ASATLGNRIGKFSFLLGVNHLENTSQPLQFATLAKSRTPAKAGDTPVTGAYFYNNQTNTP
TAVPGVNGEGIEHTVQDQFKLKMQYDFTPTLQAGFTLGYWHQTYNSQTSSFLRDANGNPV
YSGKVAIDGYEYTIPAAALAPSLGHSENWLYGVSLKTRNTTGWNGEAIASYYDVSNSVAR
TANSGAPGNGPGTVLFGDGAGWKTLDLRSTYTPATNQAGLANHALSFGYHYDNYFVDNES
YNTLNWRDGAATSFANAFAGKTQTQALYAQDAWRFLPRWKLVYGVRYEDWQAYGGSQSLG
GTTLPYSDVSQQHFSPKASLSFDVTDDLILRASIGRAYRFPTVGELFQGQINGSAIVNNN
PNLKPEDDLSKELSAEWAHWNGVFRFSLFQDDVKNTIFSQTDTTVIPNVTNFQNIGKVRS
RGVETSYSGQDVLVRGLDLLASVAYTQSKIIANAQNPASVGKYFYRIPLWRANLAATYHF
DERAALTLAARYSGRQYNTLTNTDTNPDVFGGTSSYTVADAKFSYKPTRLSEIGVGVDNL
FDARYFVYHPYPGRTFYVEAKLRM