Protein Info for BPHYT_RS22520 in Burkholderia phytofirmans PsJN

Annotation: RND transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 533 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details TIGR01845: efflux transporter, outer membrane factor (OMF) lipoprotein, NodT family" amino acids 19 to 500 (482 residues), 397.9 bits, see alignment E=3e-123 PF02321: OEP" amino acids 95 to 287 (193 residues), 94.9 bits, see alignment E=2.9e-31 amino acids 314 to 496 (183 residues), 96.8 bits, see alignment E=7.2e-32

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_4536)

Predicted SEED Role

"RND efflux system, outer membrane lipoprotein CmeC" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TDZ2 at UniProt or InterPro

Protein Sequence (533 amino acids)

>BPHYT_RS22520 RND transporter (Burkholderia phytofirmans PsJN)
MKRFESLSGWGRAAASGLLVALLAACSLEPTYKRPDVDAPAAFKEAPAASATAGASAPSA
ASGVSAQDNGTWKQAQPADDAHRGEWWTIFGDPQLNALEEQASAANQDLKAAAARVQQAR
AVTQAAKSDWFPKLDAGFGPTRERASAASQFQPDSVGGTNGTIWRAQTTASYEADLFGRV
GSNVNASRSDEQQSEALFRSVQLSLQADVAQNYFQLRELDTDQDLYRRTVALREDTLKLV
ERRFKEGDIGELDVSRARNELASARADAVGVARQRAASEHSLAILLGKPPADFSFAEAPL
VPVTVRVPPGLPSDLLERRPDISAAERAMQAANARVGLAKSAFFPKLDITGGAGFESATL
GDLFKWSSRAFILGPFAGTALTLPLFDGGRRKANLAQARSKYDEDVAQYRQQVLVAFREV
EDNLSDLRLLDDQMREQNDAVQASQRAAHLSRTQYTEGAVSYLDVIDGERQVLTSQLQAS
HLSGTQAVATVNLIRALGGGWGDVKAADTAVGAVAPASAPVTAAAAVQQVAKQ