Protein Info for BPHYT_RS22455 in Burkholderia phytofirmans PsJN

Annotation: phosphonate ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 325 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details TIGR01098: phosphate/phosphite/phosphonate ABC transporter, periplasmic binding protein" amino acids 4 to 250 (247 residues), 262.7 bits, see alignment E=3.5e-82 TIGR03431: phosphonate ABC transporter, periplasmic phosphonate binding protein" amino acids 4 to 289 (286 residues), 347.5 bits, see alignment E=6.1e-108 PF12974: Phosphonate-bd" amino acids 30 to 277 (248 residues), 245.7 bits, see alignment E=2.4e-77

Best Hits

Swiss-Prot: 51% identical to PHND_ECOLI: Phosphonates-binding periplasmic protein (phnD) from Escherichia coli (strain K12)

KEGG orthology group: K02044, phosphonate transport system substrate-binding protein (inferred from 100% identity to bpy:Bphyt_4523)

Predicted SEED Role

"Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1)" in subsystem ABC transporter alkylphosphonate (TC 3.A.1.9.1) (TC 3.A.1.9.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TGT5 at UniProt or InterPro

Protein Sequence (325 amino acids)

>BPHYT_RS22455 phosphonate ABC transporter substrate-binding protein (Burkholderia phytofirmans PsJN)
MKFLRSLITLAVGAATLATVAAAQAEDLNLGIISTDSSSVLKQRWEPLIQDMNKQTGLNV
KAFFATDYAGIIEGMRFNKVQVAYLGNASAIEAVDRSQGEVFAKTVYANGDAGYKSVLIT
NVNSRFKTLDDVFKNTKDVTLGFGDPNSTSGTLIPGYYLFAQHNTPVNTSFKTVLPSSHE
ANLLAVVNNKIDIATNNTEMLDSLKKQHPDKFAQVRVLWTSPLIPSDPIVWRKDLPQATK
DKLRNFFVNYAKTDAHEKAVMAGIFGYGGFAASSDAQLLPIRQVALFQQKQKIESDAHLS
DDDRKTQLAALDAKLSALNTAQSKQ