Protein Info for BPHYT_RS22415 in Burkholderia phytofirmans PsJN

Annotation: nitrite reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03460: NIR_SIR_ferr
  • amino acids 53 to 82 (30 residues), 24.4 bits, see alignment (E = 2e-09)
  • amino acids 378 to 428 (51 residues), 46.5 bits, 2.5e-16
• PF01077: NIR_SIR
  • amino acids 142 to 282 (141 residues), 144 bits, E=2.8e-46
  • amino acids 442 to 585 (144 residues), 48.5 bits, E=6.7e-17

Best Hits

KEGG orthology group: K00381, sulfite reductase (NADPH) hemoprotein beta-component [EC: 1.8.1.2] (inferred from 100% identity to bpy:Bphyt_4515)

Predicted SEED Role

"Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2)" in subsystem Cysteine Biosynthesis (EC 1.8.1.2)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)

KEGG Metabolic Maps

• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.2

Use Curated BLAST to search for 1.8.1.2

Sequence Analysis Tools

See B2TGS7 at UniProt or InterPro

Protein Sequence (596 amino acids)

>BPHYT_RS22415 nitrite reductase (Burkholderia phytofirmans PsJN)
MYQYTEFDQSFVRSRAAQFRDQLERWQSGKLSEDDFRPLRLQNGWYVQRHAPMLRVAIPY
GELSSRQLRMLARIAREYDEPEAEVYRLASEAQRKLGTVRLPTNYAHFTTRTNVQFNWIP
LSKAADVMDLLASVDMHGIQTSGNCIRNISCDERAGVAPDEIADPRPFAEIMRQWTTLHP
EFAFLPRKFKIAITGASEDRAATDWHDVGLRLLRNEAGELGFRVTVGGGMGRTPVIGTVL
REFLPWQHIMNYIEAVVRVYNQYGRRDNKYKARIKILVKAEGQRYIDGVEEEFRQIVEHD
GGPHTIPLAELERVSACFALPSRPSVDAPIDLEAIATSNAVALETPQFERWLQRNVSPHR
NQRLRIVTLSFKRLLQAPGDASAEQLDRVADLAERFSAGEARVTHTQNVVLPWVHVNDLL
PLWQAAREAGLASANVNLLTDMIACPGGDFCALANARSIPVAEAITERYQDLDELNDIGD
IDLHISGCINSCGHHHSGHIGILGVDKDGKEWYQVTLGGSDGSHASGRAQAGKVIGPSFS
AAEVPEVIDALLSAYLDIRAAVGGRRESFVETVRRAGIEPFRTAADRVRTLMEKTA