Protein Info for BPHYT_RS22245 in Burkholderia phytofirmans PsJN

Updated annotation (from data): transporter for D-alanine, D-lactate, and pyruvate (MctP)
Rationale: Specific phenotype: utilization of 2-Deoxyadenosine 5-monophosphate, D-Alanine, Deoxyribonucleic acid, pyruvate. The DNA and dAMP phenotypes are not explained
Original annotation: sodium:solute symporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 493 transmembrane" amino acids 12 to 30 (19 residues), see Phobius details amino acids 51 to 76 (26 residues), see Phobius details amino acids 82 to 101 (20 residues), see Phobius details amino acids 128 to 147 (20 residues), see Phobius details amino acids 161 to 180 (20 residues), see Phobius details amino acids 191 to 210 (20 residues), see Phobius details amino acids 242 to 260 (19 residues), see Phobius details amino acids 280 to 304 (25 residues), see Phobius details amino acids 324 to 352 (29 residues), see Phobius details amino acids 373 to 394 (22 residues), see Phobius details amino acids 401 to 421 (21 residues), see Phobius details amino acids 428 to 449 (22 residues), see Phobius details amino acids 461 to 484 (24 residues), see Phobius details PF00474: SSF" amino acids 45 to 436 (392 residues), 62.7 bits, see alignment E=1.5e-21

Best Hits

Swiss-Prot: 64% identical to MCTP_RHIL3: Monocarboxylate transport permease protein (mctP) from Rhizobium leguminosarum bv. viciae (strain 3841)

KEGG orthology group: K03307, solute:Na+ symporter, SSS family (inferred from 100% identity to bpy:Bphyt_4479)

Predicted SEED Role

"Acetate permease ActP (cation/acetate symporter)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T7V3 at UniProt or InterPro

Protein Sequence (493 amino acids)

>BPHYT_RS22245 transporter for D-alanine, D-lactate, and pyruvate (MctP) (Burkholderia phytofirmans PsJN)
MSDVNPVNPVAMTVFIAFFVLVTVIGFFAARWKRGDLTQLHEWGLGGRQFGTVISWFLVG
GDFYTAYTVIAVPALVYSVGAYGFFALPYTIIVYPFVFAVMPKLWKIAHAKNHITAADYV
QGEYGGKWFPAAVAITGIVATMPYIALQLVGMQVVIKGLGVTGEMPLIVAFVILALYTYA
SGLRAPAMIAFVKDIMIYIVVIAAVWLIPAKLGGYAHVFDAADTYFKAKGGATGIILKPT
QFTAYASLALGSALAAFMYPHTMTAVLSSSSANTVRKNAIFLPAYTLLLGLIALLGYMAI
AAGVHVKSASDMVPALFNTLFPSWFVGFAAAAIAISALVPAAIMSIGAANLFTRNLWRPL
VSPNISPEGEASTAKIVSLVVKFGALLFIVFLPTQYAIDLQLLGGVWILQIFPAIVFSLY
TRRLNTPGLFLGWLAGIVLGTGLAISQGLKPVFALHLGDAVYPVYIGLIALTVNIVVSFV
VSVLSPRRVVAAA