Protein Info for BPHYT_RS22235 in Burkholderia phytofirmans PsJN

Annotation: sensor histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 471 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details transmembrane" amino acids 208 to 230 (23 residues), see Phobius details PF08269: dCache_2" amino acids 44 to 212 (169 residues), 113.1 bits, see alignment E=3.6e-36 PF17200: sCache_2" amino acids 47 to 194 (148 residues), 132.6 bits, see alignment E=3.1e-42 PF17201: Cache_3-Cache_2" amino acids 82 to 194 (113 residues), 45.5 bits, see alignment E=1.5e-15 PF07730: HisKA_3" amino acids 253 to 321 (69 residues), 46.3 bits, see alignment E=1.2e-15 PF02518: HATPase_c" amino acids 367 to 456 (90 residues), 46.9 bits, see alignment E=8.4e-16

Best Hits

KEGG orthology group: K02480, two-component system, NarL family, sensor kinase [EC: 2.7.13.3] (inferred from 100% identity to bpy:Bphyt_4477)

Predicted SEED Role

"Sensor histidine kinase"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T7V1 at UniProt or InterPro

Protein Sequence (471 amino acids)

>BPHYT_RS22235 sensor histidine kinase (Burkholderia phytofirmans PsJN)
MKLKAKIVLLAIVPFLAAIASIEVGVQRQATALAETQHASTQAAYMASKEIELKHYVELA
TTAIAPLYDAGQDNARDDATLRARALDVLQKMDFGKDGYFFVYDMHGRSLMHPREPDLVG
RDLWELRDPQGTLTIQQLLAAAARGGGYVRYLWHRPSTGKVASKLGYVVPLERWGWMIGT
GIYLDDVDNTLAHIDERAAANIDRTMKWIDAIAVAGLAVIALCALVLNVTEYRSADAKLK
RLAQQVVESQENERARLSRELHDGISQMMVSVKLLLESALARFERSEVRVPAAEAALTTS
LTRLGDTLREVRRISHALRPSMLDDLGVAAALEQLSRELSEQSGIEIGFTQIAHTHAAAL
PDAVNTVLFRIAQEALTNIVRHAQATNAALALEISNDGVTLNIADNGRGFDVAHAFVGRR
SGVGLRNMRERLEALGGTLSLSSQAGHTLVTARVPLGSTEPTLPALQETSP