Protein Info for BPHYT_RS21990 in Burkholderia phytofirmans PsJN

Annotation: FAD-dependent oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01266: DAO
  • amino acids 8 to 348 (341 residues), 238.6 bits, E=2.3e-74
• PF13450: NAD_binding_8
  • amino acids 11 to 55 (45 residues), 25.4 bits, 2.1e-09

Best Hits

KEGG orthology group: K00303, sarcosine oxidase, subunit beta [EC: 1.5.3.1] (inferred from 100% identity to bpy:Bphyt_4426)

Predicted SEED Role

"Sarcosine oxidase beta subunit (EC 1.5.3.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.5.3.1)

MetaCyc Pathways

• glycine betaine degradation I (6/8 steps found)
• creatinine degradation II (2/5 steps found)
• creatinine degradation I (1/4 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.5.3.1

Use Curated BLAST to search for 1.5.3.1

Sequence Analysis Tools

See B2TFD1 at UniProt or InterPro

Protein Sequence (375 amino acids)

>BPHYT_RS21990 FAD-dependent oxidoreductase (Burkholderia phytofirmans PsJN)
MSNTLHYDVAIAGGGLVGSSAALALAKRGLRVGLFERRYCGAQASGVNYGGVRCQGRPVE
QMPLAIRARRIWDRLPELIGIDGEFTVSGHLRLARSESDLAALQTWADMARDFGLHAQLV
SGAAFRQRYPWLGPAAVGGSLCATDGHANPRLVSPAFARAARAAGADVREQTELTDIHHD
GTRFQLRAGDLRVSADWLINSAGAWANTVAGYFGETAPMKPIYPNMWVTEPLPLFITHNL
GVYGGGIYARQVARGNCVIGGGRGHGDGEYGQPSTDTTRAVMRDACALLPALREALLIRT
WSGVEGETPDSNPIIGASRTVPRLLHAFGFSGGGFLLAPGVGEVLADLVADGATVTPLDA
FSIGRFAAVATSPVA