Protein Info for BPHYT_RS21950 in Burkholderia phytofirmans PsJN

Annotation: RND transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 477 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR01845: efflux transporter, outer membrane factor (OMF) lipoprotein, NodT family" amino acids 13 to 463 (451 residues), 282.2 bits, see alignment E=3.7e-88 PF02321: OEP" amino acids 66 to 249 (184 residues), 77.6 bits, see alignment E=5.7e-26 amino acids 276 to 461 (186 residues), 75.3 bits, see alignment E=2.9e-25

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_4418)

Predicted SEED Role

"Outer membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TFC3 at UniProt or InterPro

Protein Sequence (477 amino acids)

>BPHYT_RS21950 RND transporter (Burkholderia phytofirmans PsJN)
MKPILVKVLASAALLTLAACAVQPETHADLPQTVKTVAPAAWNVDAPQDSVSANAWWDQF
GDPVMHQLVESVLTGNLDVQAAVERVRQAQDLAKQNRAALLPELNASATASDSRQNTPPP
LGYVRQAGVGLTASWTPDVFGGERLAVLAAQAQVSGRQSALNEVRLALAANTAAAYIDLR
WAQSQLQILSDNEQIRARALKLTQQRLHYGLSTQLDVARAQNQLQDLQAQIPRVQSAVQH
QLSLIAVYSGRTPESVDSLLLANARDIPVPAQSVPQMLPSEALLQRPDVRTAYATVEQRA
AEVGVSKAQRYPQFRLNLADGLLASSYLGLPTLTDNLFSAALNATSPIFNAGRITANIDA
SESRMRESQLGLQQTMLQALKEIEDNRSDLVSGAVQVQRLGGALDASNQALHLSSELYKG
GAADFLDVLTAQEAYLRDAESLNQAKREHALSAVALYRSLGGGWDVPDAVTAVSSSN