Protein Info for BPHYT_RS21115 in Burkholderia phytofirmans PsJN
Annotation: radical SAM protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 48% identical to COFH_METJA: 5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase (cofH) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
KEGG orthology group: K11779, FO synthase [EC: 2.5.1.-] (inferred from 100% identity to bpy:Bphyt_4252)MetaCyc: 48% identical to 5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-methylphenol transferase (Methanocaldococcus jannaschii)
RXN-19229 [EC: 2.5.1.147]
Predicted SEED Role
"7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2" in subsystem Coenzyme F420 synthesis
MetaCyc Pathways
- factor 420 biosynthesis I (archaea) (3/5 steps found)
- 3PG-factor 420 biosynthesis (2/5 steps found)
- factor 420 biosynthesis II (mycobacteria) (2/6 steps found)
KEGG Metabolic Maps
- Biosynthesis of terpenoids and steroids
- Carotenoid biosynthesis - General
- Methionine metabolism
- Porphyrin and chlorophyll metabolism
- Riboflavin metabolism
- Terpenoid biosynthesis
- Tryptophan metabolism
- Ubiquinone and menaquinone biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.5.1.-
Use Curated BLAST to search for 2.5.1.- or 2.5.1.147
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See B2TEW0 at UniProt or InterPro
Protein Sequence (423 amino acids)
>BPHYT_RS21115 radical SAM protein (Burkholderia phytofirmans PsJN) MAARRNGDDPSALLSAASAPVRFILSRHLEGAEISRDEGRVLFEADGEDLKAVVATADHV RRERVGDVVSFVVTRNINFTNVCYMGCRFCGFAKRREEEGAELLSMDEVARRADVARQRG ATEVCIQGGLHPDLTDHHYLDILAAIKARVPEMHIHAFSPFEIWYGSKKRRQAAADYLRE LKAAGLGSIPGTAAEILDTDVRRQLTRNKLSAQAWVEIVRAAHGVGLRSTSTMMYGHVDG PGHWAAHIDLLRSIQKETGGFTEFVPLGFVHSEAPLYMEHEIPGVRAGATLVEHIKVHAI ARLMLAGWIDNIQVSWVKLGPQTGQALLAAGANDFGGTLMDESISRSAGASFGEEITAAE MVSIIRDAGREPVRRSTLYVPLERYVDHDPVALPPLKARAYDPIRFLRNRPQAGCDNEEL AHA