Protein Info for BPHYT_RS21110 in Burkholderia phytofirmans PsJN

Annotation: FO synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 386 TIGR03550: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase, CofG subunit" amino acids 38 to 347 (310 residues), 431.4 bits, see alignment E=1e-133 PF04055: Radical_SAM" amino acids 45 to 222 (178 residues), 39.1 bits, see alignment E=4.5e-14

Best Hits

Swiss-Prot: 48% identical to COFG_METKA: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase (cofG) from Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)

KEGG orthology group: K11779, FO synthase [EC: 2.5.1.-] (inferred from 100% identity to bpy:Bphyt_4251)

Predicted SEED Role

"7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 1" in subsystem Coenzyme F420 synthesis

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.-

Use Curated BLAST to search for 2.5.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TEV9 at UniProt or InterPro

Protein Sequence (386 amino acids)

>BPHYT_RS21110 FO synthase (Burkholderia phytofirmans PsJN)
MLSRAEAIALDQVSAGELHQLMRRACRVREKAWGATVTYSRKVFIPLTNLCRDKCGYCTF
ARQPDEPGAGYLAPDQVMAIARRGEALGCKEALFSLGEKPELVYPQARAVLDALGYATTM
DYVIAMSERILAETSLIPHVNAGTLTRAEIVRLKAVCGSVGLMLENVSRRLVQRGMAHYA
CPDKVPVQRLRTLDMAGVERIATTTGILIGIGETWVERIDSLLAIAELHTRHGHIQEVIV
QNFRAKPGTMMATWNEPGHEDMLRTLAVARLLLPTDISLQAPPNLAEAFEDYLDSGINDW
GGISPVTADYINPERAWPAIHDVARRSHGRGLGLAERLTVYPRYLCQPDFVGAAPARALG
CQARPDGLALHQYEAMPMAASAECAA