Protein Info for BPHYT_RS20800 in Burkholderia phytofirmans PsJN
Annotation: alanine-phosphoribitol ligase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 57% identical to SDH_GLUOY: L-sorbose 1-dehydrogenase from Gluconobacter oxydans
KEGG orthology group: K00119, [EC: 1.1.99.-] (inferred from 100% identity to bpy:Bphyt_4183)MetaCyc: 57% identical to L-sorbose dehydrogenase, FAD dependent (Gluconobacter oxydans UV10)
L-sorbose 1-dehydrogenase. [EC: 1.1.99.32]
Predicted SEED Role
"Choline dehydrogenase (EC 1.1.99.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.1.99.1)
MetaCyc Pathways
- 2-keto-L-gulonate biosynthesis (4/4 steps found)
- choline-O-sulfate degradation (3/3 steps found)
- choline degradation I (2/2 steps found)
- glycine betaine biosynthesis I (Gram-negative bacteria) (2/2 steps found)
- glycine betaine biosynthesis II (Gram-positive bacteria) (2/2 steps found)
- L-ascorbate biosynthesis III (D-sorbitol pathway) (3/4 steps found)
KEGG Metabolic Maps
- Aminophosphonate metabolism
- Benzoate degradation via hydroxylation
- Glycine, serine and threonine metabolism
- Limonene and pinene degradation
Isozymes
Compare fitness of predicted isozymes for: 1.1.99.-, 1.1.99.1
Use Curated BLAST to search for 1.1.99.- or 1.1.99.1 or 1.1.99.32
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See B2TEP4 at UniProt or InterPro
Protein Sequence (557 amino acids)
>BPHYT_RS20800 alanine-phosphoribitol ligase (Burkholderia phytofirmans PsJN) MYDYVIVGGGAAGCALAARLTEDAGNRVLLLEAGPADTDPYIHMPVGFFKMTGGPLTWGY RTTAAEHTQRRQIPFAQGRVLGGGGSINAMVYTRGQPADYDGWERDGCTGWGFRDGVLPY LRRMEDNERLCNEYHGVGGPLGVSDLISVNELTKAFVLAGQEAGMPYNSDFNGAQQEGVG VYQVTQRSGKRCSAAVGYLRDARSRPNLTVRTDCLVSRIVIEKGRAVGVEFAPRGERANV TIARAEREVIVTAGAIGSPKLLMLSGIGRAEDLERVGVKPVHALNGVGQNLQDHFDIDIV YELNGPYSLDKYAKKHMMLLAGLEYKLFNKGPVTSNIAEGGAFWYSDSSVPTPDLQFHFL PGAGVEAGVPPVPSGSGCTLNSYFLRPRSRGSVTLHSADPADAPLIDPAYIRDPYDLKVA VDGIRQSREIMSQHALRKYIRSEHFPGGLVRTQAEYETYAQQYGRTGYHPVGTCKMGVDE MSVVDPQLRVRGIEGLRVADSSVMPRIVSSNTNAPTLMIAEKAADLIRGLAAQPAAVKGA LASTSGRVARESAAAAV