Protein Info for BPHYT_RS20785 in Burkholderia phytofirmans PsJN

Annotation: CoA-transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01144: CoA_trans
  • amino acids 3 to 237 (235 residues), 77.9 bits, E=3.7e-26

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_4180)

MetaCyc: 42% identical to propanoyl-CoA transferase monomer (Megasphaera elsdenii)
Propionate CoA-transferase. [EC: 2.8.3.1]; 2.8.3.1 [EC: 2.8.3.1]; 2.8.3.1 [EC: 2.8.3.1]; 2.8.3.1 [EC: 2.8.3.1]

Predicted SEED Role

"Acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (EC 2.8.3.8)" in subsystem Acetyl-CoA fermentation to Butyrate (EC 2.8.3.8)

MetaCyc Pathways

• acetyl-CoA fermentation to butanoate (6/7 steps found)
• acetoacetate degradation (to acetyl CoA) (2/2 steps found)
• 1,2-propanediol biosynthesis from lactate (engineered) (4/6 steps found)
• L-alanine degradation VI (reductive Stickland reaction) (4/6 steps found)
• propanoate fermentation to 2-methylbutanoate (4/6 steps found)
• superpathway of L-alanine fermentation (Stickland reaction) (6/9 steps found)
• pyruvate fermentation to acetone (3/5 steps found)
• (R)-lactate fermentation to propanoate II (acrylate pathway) (2/4 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (8/13 steps found)
• 4-aminobutanoate degradation V (3/7 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (5/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (10/17 steps found)
• gallate degradation III (anaerobic) (5/11 steps found)
• L-glutamate degradation XI (reductive Stickland reaction) (2/7 steps found)
• succinate fermentation to butanoate (2/7 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Propanoate metabolism
• Pyruvate metabolism
• Styrene degradation

Isozymes

Compare fitness of predicted isozymes for: 2.8.3.8

Use Curated BLAST to search for 2.8.3.1 or 2.8.3.8

Sequence Analysis Tools

See B2TEP1 at UniProt or InterPro

Protein Sequence (496 amino acids)

>BPHYT_RS20785 CoA-transferase (Burkholderia phytofirmans PsJN)
MDAAAAAEQIFDGATIALAGSGGGLLEPDAVLAALEQRFLQTGHPRDLTVVHALGIGDGK
SSGLSRFAHAGMVRRVIGGHWSWSPAMQQMAKDNAFEAYSFPAGAISTLLREIGAGRPGL
VTHVGLRTFVDPRLDGGKINARASEELVELIELDGREYLRYKPFKVDFAIVRGSSADANG
NVTLRREPADLDTYAAALAAHNSGGRVIVQVKERTTQGYVPARLVRIPGILVDTLVAARE
QVQCVVADYDPALSGESLSPIDDGFYETPTGIRYIIAARAAQELHAGQSANFGFGIPGGI
PGVLAQQGRLGTFWGSVEQGIHNGAMLDGPMFGTARNAEAILSSVDQFDFYSGGGVDITF
LGMGEMDGEGNINVSKLGATVVGPGGFIDITQGARKIVFCGSFEAKGLQVEKAGAGVKIV
SHGSVPKLVERVQHVTFSGEQARIAKQEVLYVTERAVFRLEESGVRLIEVAQGVDVERDV
LARMSFRPLVDEALLA