Protein Info for BPHYT_RS20415 in Burkholderia phytofirmans PsJN
Annotation: 5-dehydro-4-deoxyglucarate dehydratase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 79% identical to KDGD_PSEPU: 5-dehydro-4-deoxyglucarate dehydratase from Pseudomonas putida
KEGG orthology group: K01707, 5-dehydro-4-deoxyglucarate dehydratase [EC: 4.2.1.41] (inferred from 100% identity to bpy:Bphyt_4103)MetaCyc: 70% identical to 5-dehydro-4-deoxyglucarate dehydratase subunit (Acinetobacter baylyi ADP1)
5-dehydro-4-deoxyglucarate dehydratase. [EC: 4.2.1.41]
Predicted SEED Role
"5-dehydro-4-deoxyglucarate dehydratase (EC 4.2.1.41)" in subsystem D-Galacturonate and D-Glucuronate Utilization or D-galactarate, D-glucarate and D-glycerate catabolism (EC 4.2.1.41)
MetaCyc Pathways
- D-galactarate degradation II (3/3 steps found)
- D-glucarate degradation II (3/3 steps found)
- D-glucuronate degradation II (4/5 steps found)
- D-galacturonate degradation II (3/5 steps found)
- superpathway of microbial D-galacturonate and D-glucuronate degradation (20/31 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.2.1.41
Use Curated BLAST to search for 4.2.1.41
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See B2TEG6 at UniProt or InterPro
Protein Sequence (305 amino acids)
>BPHYT_RS20415 5-dehydro-4-deoxyglucarate dehydratase (Burkholderia phytofirmans PsJN) MTSPQELKAIVSEGLLSFPVTDFDAHGEFRADTYAARLEWLAPYGATALFAAGGTGEFFS LTKTDYTNVIRTATDTCRGKVPILAGAGGPTRVAIEYAQEAERLGASGVLLMPHYLTEAS QEGIAAHVEQVCKSVPNIGVIVYNRANSKLNADMLERLADRCPNLIGFKDGVGEIEAMVT IRRRLGERFSYLGGLPTAEVYAAAYKALGVPVYSSAVFNFIPKTAMEFYRAVAANDHATQ GRLLDEFFLPYLAIRNRRAGYAVSIVKAGAKLVGHDAGPVRAPLTDLTDEESAQLDALIR KLGAQ