Protein Info for BPHYT_RS20410 in Burkholderia phytofirmans PsJN

Annotation: glucarate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 449 TIGR03247: glucarate dehydratase" amino acids 10 to 449 (440 residues), 882.3 bits, see alignment E=2.7e-270 PF02746: MR_MLE_N" amino acids 45 to 141 (97 residues), 25.3 bits, see alignment E=1.6e-09 PF13378: MR_MLE_C" amino acids 192 to 408 (217 residues), 158 bits, see alignment E=2.9e-50

Best Hits

Swiss-Prot: 78% identical to GUDD_PSEPU: Glucarate dehydratase (gudD) from Pseudomonas putida

KEGG orthology group: K01706, glucarate dehydratase [EC: 4.2.1.40] (inferred from 100% identity to bpy:Bphyt_4102)

MetaCyc: 78% identical to D-glucarate dehydratase subunit (Pseudomonas putida)
Glucarate dehydratase. [EC: 4.2.1.40]

Predicted SEED Role

"Glucarate dehydratase (EC 4.2.1.40)" in subsystem D-galactarate, D-glucarate and D-glycerate catabolism (EC 4.2.1.40)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.40

Use Curated BLAST to search for 4.2.1.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TEG5 at UniProt or InterPro

Protein Sequence (449 amino acids)

>BPHYT_RS20410 glucarate dehydratase (Burkholderia phytofirmans PsJN)
MSTKSSELNATPVVTELRVVPVAGRDSMLLNLSGAHGPFFTRNIVILRDSAGHTGVGEVP
GGESIRKTIDDARPLVVGQSIGNLQAVLNKARTQFADRDAGGRGLQTFDLRTTIHAVTAL
EAALLDLLGQHLGVPVAALLGEGQQRDEVEMLGYLFYIGDRKKTDLEYASGADGRDDWER
VRTEEAMTPEAVVRLAEAAQARYGFNDFKLKGGVLPGDAEIEAVTALAERFPKARVTLDP
NGAWSLAEAVRLCRDQHDVLAYAEDPCGAENGYSGREVMAEFRRATGLPTATNMIATDWR
QMGHAIQLQSVDIPLADPHFWTMQGSVRVAQMCNDWGLTWGSHSNNHFDISLAMFTHVAA
AAPGKITAIDTHWIWQDGQRLTRDPLRIVGGKVKVPQAAGLGIELDMDEIEKAHALYQQH
GLGARDDGVAMQYLIPNWKFDNKRPCLVR