Protein Info for BPHYT_RS20315 in Burkholderia phytofirmans PsJN

Annotation: major facilitator transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 433 transmembrane" amino acids 27 to 45 (19 residues), see Phobius details amino acids 66 to 85 (20 residues), see Phobius details amino acids 97 to 115 (19 residues), see Phobius details amino acids 122 to 145 (24 residues), see Phobius details amino acids 156 to 178 (23 residues), see Phobius details amino acids 190 to 212 (23 residues), see Phobius details amino acids 258 to 279 (22 residues), see Phobius details amino acids 291 to 312 (22 residues), see Phobius details amino acids 324 to 343 (20 residues), see Phobius details amino acids 349 to 371 (23 residues), see Phobius details amino acids 382 to 406 (25 residues), see Phobius details amino acids 412 to 432 (21 residues), see Phobius details PF07690: MFS_1" amino acids 35 to 400 (366 residues), 164.4 bits, see alignment E=1.8e-52

Best Hits

Swiss-Prot: 43% identical to NICT_PSEPK: Putative metabolite transport protein NicT (nicT) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_4083)

Predicted SEED Role

"Nitrate/nitrite transporter" in subsystem Nitrate and nitrite ammonification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TEE6 at UniProt or InterPro

Protein Sequence (433 amino acids)

>BPHYT_RS20315 major facilitator transporter (Burkholderia phytofirmans PsJN)
MHGYSHSDATTSGGNTSVGVERTYRKIFFRLIPLLFLCYVISYIDRVNISFAKLQFMHDL
GFSESTYGLGAGLFFIGYVLFEVPSNLWMQRIGARRTLLRIMFAWGLVSSLMMFVRTPMQ
FYAMRFLLGVTEAGFFPGIIFYLSCWFPGARRARVTAFLMMATAVAGIVGGPMSGFIMTR
LAGVGGLPGWQWLFLLEGVPAVTLGIVAFLLLGDKPDDVSWLDEHEKALVSRELAADEKA
KQTGHLDGLRGALANPRVYLAALVYFAVMMPFNAIGFWAPTIIRDLGVANVLDVGLLSAL
VFIAAALGTYVVGASSDRTMERRWHVAVSACVTALAFALLPAAGHNVTLGVGLLALAAAA
SYGSFVVFWTIPQTFLPRSSAAGGIAMITSLGGTGGFVSPAFVGWVKTQTGSAQYGLIGL
AVVLLAGVVLMLV