Protein Info for BPHYT_RS19980 in Burkholderia phytofirmans PsJN

Annotation: citrate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00285: Citrate_synt
  • amino acids 10 to 65 (56 residues), 29.2 bits, E=2.6e-11
  • amino acids 58 to 250 (193 residues), 101.8 bits, E=2.2e-33

Best Hits

KEGG orthology group: K01647, citrate synthase [EC: 2.3.3.1] (inferred from 100% identity to bpy:Bphyt_4013)

Predicted SEED Role

"Citrate synthase (si) (EC 2.3.3.1)" in subsystem Serine-glyoxylate cycle or TCA Cycle (EC 2.3.3.1)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• glyoxylate cycle (6/6 steps found)
• TCA cycle V (2-oxoglutarate synthase) (8/9 steps found)
• mixed acid fermentation (13/16 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• TCA cycle II (plants and fungi) (7/9 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (7/9 steps found)
• TCA cycle VII (acetate-producers) (7/9 steps found)
• nitrogen remobilization from senescing leaves (6/8 steps found)
• ethene biosynthesis V (engineered) (18/25 steps found)
• TCA cycle III (animals) (7/10 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• methylaspartate cycle (11/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.3.1

Use Curated BLAST to search for 2.3.3.1

Sequence Analysis Tools

See B2TE78 at UniProt or InterPro

Protein Sequence (274 amino acids)

>BPHYT_RS19980 citrate synthase (Burkholderia phytofirmans PsJN)
MTQAFDAAKLAADYWSTSIIDIHPGSINVRGFPIQELIGNISFPQMIWLMLRGELPDTEQ
ARLFEAALVSSVDHGPHAPSIAISRMATSCGLPLNGAMASAINALDDVHGGAGQQAVELY
DAIDVRMQAGATLEHAVEQGVDAFIAAHGKYLPGFGHRFHPVDPRAPRLLALVDENVRAE
RISGRYAVIARGIEALLMSRKSKPVPMNIDGATAVIYAELGFAPELARGVFCLSRAVGIL
SHAWEQRGRKERNKGPMPRQMAYTYTGKPERHLP