Protein Info for BPHYT_RS19830 in Burkholderia phytofirmans PsJN

Annotation: tRNA modification GTPase TrmE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 464 PF10396: TrmE_N" amino acids 9 to 130 (122 residues), 123.3 bits, see alignment E=1.2e-39 TIGR00450: tRNA modification GTPase TrmE" amino acids 13 to 464 (452 residues), 332.2 bits, see alignment E=5.9e-103 PF12631: MnmE_helical" amino acids 133 to 461 (329 residues), 193.5 bits, see alignment E=1.1e-60 PF02421: FeoB_N" amino acids 228 to 379 (152 residues), 47.9 bits, see alignment E=2.1e-16 TIGR00231: small GTP-binding protein domain" amino acids 228 to 377 (150 residues), 82.3 bits, see alignment E=3.5e-27 PF01926: MMR_HSR1" amino acids 229 to 342 (114 residues), 90.8 bits, see alignment E=1.3e-29

Best Hits

Swiss-Prot: 97% identical to MNME_PARXL: tRNA modification GTPase MnmE (mnmE) from Paraburkholderia xenovorans (strain LB400)

KEGG orthology group: K03650, tRNA modification GTPase (inferred from 100% identity to bpy:Bphyt_3984)

Predicted SEED Role

"GTPase and tRNA-U34 5-formylation enzyme TrmE" in subsystem Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T7T9 at UniProt or InterPro

Protein Sequence (464 amino acids)

>BPHYT_RS19830 tRNA modification GTPase TrmE (Burkholderia phytofirmans PsJN)
MLTTDSDPIVAIATAPGRGGIGVVRISFGRAGEAAAQPLMQALTGQTLAPRHASYVPFLD
VSGNALDRGIALYFPAPHSYTGEHVLELQGHGGPVVLQLVLQRCIDAGRAFGLRLAEPGE
FTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDGAFSRDIHALVEDVITLRMLV
EATLDFPEEEIDFLEAADARGKLTRIRERLAHVLSEARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQIEGIPLHVIDTAGLRDTEDEVEKIGIARTW
SEIERADVVLHLLDARTGMTAEDDAIAGRFPRGVPVVRVLNKTDLTGLAPATQALDADLE
LSEVRLSAKQGDGVALLRDELLRIAGWQAGAESVYLARERHLIALRAAEEHLATAAAHAD
QNSQALDLFAEELRLAQDQLNSITGEFSSDDLLGVIFSRFCIGK