Protein Info for BPHYT_RS19680 in Burkholderia phytofirmans PsJN

Annotation: glutamate--cysteine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 538 TIGR01434: glutamate--cysteine ligase" amino acids 15 to 528 (514 residues), 631.1 bits, see alignment E=6.8e-194 PF04262: Glu_cys_ligase" amino acids 18 to 391 (374 residues), 531.8 bits, see alignment E=3.6e-164

Best Hits

Swiss-Prot: 62% identical to GSH1_PSEA7: Glutamate--cysteine ligase (gshA) from Pseudomonas aeruginosa (strain PA7)

KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 100% identity to bpy:Bphyt_3953)

Predicted SEED Role

"Glutamate--cysteine ligase (EC 6.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 6.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.2.2

Use Curated BLAST to search for 6.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T7Q9 at UniProt or InterPro

Protein Sequence (538 amino acids)

>BPHYT_RS19680 glutamate--cysteine ligase (Burkholderia phytofirmans PsJN)
MPNTTPSRTTDAFSHRLSVLTSGPQRDALIRGLRGIEKESLRVTHEGKLALTPHSRALGS
ALTHPSLTTDYSEALLELITPAEHDVALTLEKLDTLHRFVYAQLGDEILWNNSMPGLLPD
TDDGIPIAHYGTSNIGKLKYVYRIGLALRYGRTMQCIAGIHYNYSLNEEVWRLLHADQQS
TANAVDFQSDRYLALIRNFRRTNWLLMYLFGASPALDRRFLRDRKHTLDSLDADTLYRPY
ATSLRMSDLGYSNTTAQAELHADYDTLPGYLDALAKAVSQPYPAYEAIGTQRDGEWVQIN
TNVLQIENEFYSTIRPKRITHPGERPLHALAARGVQYVEVRCMDIDPFEPTGISLETSRF
LDAYLLVCALDDSALLPPDAYAEANQNFGRVTMEGRKPGLELTRDGQPVAMTDWANELLI
KIDAAAAALDTLHGGDAHARAVAVQRAKLADASLTPSARVLQTMREKQQSFLAFGLEQSE
AHAAYFRARPLDAEQTQAFTDLAAQSLAEQAKLEQEEVGSFDAFVAAYRAYTLNRFSV