Protein Info for BPHYT_RS19370 in Burkholderia phytofirmans PsJN

Annotation: arogenate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00497: SBP_bac_3
  • amino acids 45 to 264 (220 residues), 143.3 bits, E=4.2e-46

Best Hits

Swiss-Prot: 52% identical to PHEC_PSEAE: Cyclohexadienyl dehydratase (pheC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_3891)

MetaCyc: 52% identical to arogenate dehydratase (Pseudomonas aeruginosa PAO1)
Arogenate dehydratase. [EC: 4.2.1.91]; Prephenate dehydratase. [EC: 4.2.1.91, 4.2.1.51]

Predicted SEED Role

"Cyclohexadienyl dehydratase (EC 4.2.1.51)(EC 4.2.1.91)" in subsystem Phenylalanine and Tyrosine Branches from Chorismate (EC 4.2.1.51, EC 4.2.1.91)

MetaCyc Pathways

• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-phenylalanine biosynthesis (10/10 steps found)
• L-phenylalanine biosynthesis I (3/3 steps found)
• L-phenylalanine biosynthesis III (cytosolic, plants) (2/2 steps found)
• L-phenylalanine biosynthesis II (3/4 steps found)
• superpathway of chorismate metabolism (42/59 steps found)
• superpathway of L-phenylalanine and L-tyrosine biosynthesis (3/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.51

Use Curated BLAST to search for 4.2.1.51 or 4.2.1.91

Sequence Analysis Tools

See B2T7J7 at UniProt or InterPro

Protein Sequence (265 amino acids)

>BPHYT_RS19370 arogenate dehydratase (Burkholderia phytofirmans PsJN)
MNKAALALLGTLAGVLMHAGVAGAQTSATTATPSRLDEILARGTLRACTTGDYKPYSFYK
ADGQFEGIDIDMTESLAKSLGVKTEYIKTSWSNLMNDFVAKCDVGVGGVSPTLERQKRAF
FTQAYMVDGKTPIVRCDDVNKYQTVAQIDQPATRVIVNPGGTNERFAKQYFPHANLTVYP
DNVTIFKQILAGKADVMVTDASETLLQQKLNPGLCSVHPDKPFQYGEKAWLLPRGDVAFQ
QYVDQWLHLARATGEYQAISDKWLK