Protein Info for BPHYT_RS19355 in Burkholderia phytofirmans PsJN

Annotation: bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF21775: PutA_1st
  • amino acids 11 to 43 (33 residues), 61.3 bits, see alignment (E = 1.1e-20)
• PF18327: PRODH
  • amino acids 90 to 137 (48 residues), 66.2 bits, see alignment (E = 6.5e-22)
• PF14850: Pro_dh-DNA_bdg
  • amino acids 145 to 256 (112 residues), 156.9 bits, E=6.2e-50
• PF01619: Pro_dh
  • amino acids 266 to 563 (298 residues), 355.8 bits, E=5e-110
• TIGR01238: delta-1-pyrroline-5-carboxylate dehydrogenase
  • amino acids 600 to 1099 (500 residues), 792.6 bits, E=6.4e-243
• PF00171: Aldedh
  • amino acids 653 to 1095 (443 residues), 366.3 bits, E=5.3e-113

Best Hits

KEGG orthology group: K13821, proline dehydrogenase / delta 1-pyrroline-5-carboxylate dehydrogenase [EC: 1.5.1.12 1.5.99.8] (inferred from 65% identity to aav:Aave_3321)

MetaCyc: 71% identical to proline dehydrogenase/1-pyrroline-5-carboxylate dehydrogenase (Pseudomonas putida KT2440)
RXN-14903 [EC: 1.5.5.2]; 1.5.5.2 [EC: 1.5.5.2]; RXN-14116 [EC: 1.5.5.2, 1.2.1.88]

Predicted SEED Role

"Transcriptional repressor of PutA and PutP / Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)" in subsystem Arginine and Ornithine Degradation or Proline, 4-hydroxyproline uptake and utilization or Respiratory dehydrogenases 1 (EC 1.5.1.12, EC 1.5.99.8)

MetaCyc Pathways

• L-proline degradation I (3/3 steps found)
• proline to cytochrome bo oxidase electron transfer (2/2 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-N^δ-acetylornithine biosynthesis (4/7 steps found)
• L-arginine degradation I (arginase pathway) (1/3 steps found)
• ethene biosynthesis II (microbes) (1/4 steps found)
• (5R)-carbapenem carboxylate biosynthesis (1/6 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.5.1.12

Use Curated BLAST to search for 1.2.1.88 or 1.5.1.12 or 1.5.5.2 or 1.5.99.8

Sequence Analysis Tools

See B2T7J3 at UniProt or InterPro

Protein Sequence (1309 amino acids)

>BPHYT_RS19355 bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase (Burkholderia phytofirmans PsJN)
MASTTLGVKVDDLLRSRLKDAATRLERTPHWLIKQAIFAYLEKIEHGQLPPELSGVTGSA
DLADGASVEQEEDGASHPFLEFAQNVQPQSVLRAAITAAYRRPEPECLPFLVGQARLPAN
LAGDVQTMAGKLVETLRTKSKGGGVEGLIHEFSLSSQEGVALMCLAEALLRIPDRATRDA
LIRDKISKGDWKSHMGQAPSMFVNAATWGLMITGKLVTTNSETSLSSALTRLIGKGGEPL
IRKGVDMAMRLMGEQFVTGENISEALANSRKYEARGFRYSYDMLGEAATTEADAQRYYAS
YEQAIHAIGKAAGGRGIYEGPGISIKLSALHARYSRSQQERTMSELLPRVRSLAILARRY
DIGLNIDAEEADRLEISLDLLEALCFDPELAGWNGIGFVVQAYQKRCPFVIDYIIDLARR
SRHRIMVRLVKGAYWDTEIKRAQVDGLEGYPVYTRKIYTDVSYLACAKKLLSAPDAVYPQ
FATHNAHTLSAIYHLAGNNYYPGQYEFQCLHGMGEPLYEEVTGRDKLNRPCRVYAPVGTH
ETLLAYLVRRLLENGANTSFVNRIADENVAIKDLIADPVDEASKIVPLGAPHAKIPLPRN
LYGAERLNSMGLDLSNEHRLASLSSALLASAHHPWRAAPMLEDNEIAVGVARDVRNPADH
RDLVGTVVEATPEHVSAALAHAVAAAPIWQATPVDARADCLARAADLLEAQMHTLMGLVV
REAGKSLANAVAEIREAIDFLRYYSTQIRDEFSNDTHRPLGPVVCISPWNFPLAIFMGQV
AAALAAGNTVLAKPAEQTPLIAAQAVRILREAGVPAGAVQLLPGNGETVGAALVADPRTR
AVMFTGSTEVARLINKTLSGRLDPDGKPIPLIAETGGQNAMIVDSSALAEQVVADVLQSS
FDSAGQRCSALRVLCLQDDVADRTLEMLTGAMRELAVGNPDRLSIDVGPVIDLDAKRGID
AHVATMREKGRKVEQLPMPDGCAQGTFVPPTLIELDSIDELKREVFGPVLHVVRYRRSQL
DKLLEQIRTTGYGLTLGIHTRIDETIAHVISRAHVGNIYVNRNVIGAVVGVQPFGGEGLS
GTGPKAGGALYLQRLLATRPAGLPKSLAQALVADAPNAAENSDNPSAALTAYRDWLIAEQ
QPVLAARCDGYLSHMPAGATAVLSGPTGERNTYTLGPRGTVLCIASTASGARVQFAAALA
TGNRALFEGAAGEQLVAQLPASLKSRASVKKSADATFDAVLFEGDSDELLALVKEVAKRP
GPIVSVQGVAARALESGDEDYALERLLTERSVSVNTAAAGGNANLMTIG