Protein Info for BPHYT_RS19330 in Burkholderia phytofirmans PsJN

Updated annotation (from data): glycine cleavage system P protein (EC 1.4.4.2)
Rationale: Specific phenotype: utilization of Gly-Glu
Original annotation: glycine dehydrogenase (decarboxylating)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 978 transmembrane" amino acids 344 to 364 (21 residues), see Phobius details PF02347: GDC-P" amino acids 28 to 454 (427 residues), 602.2 bits, see alignment E=8.8e-185 amino acids 494 to 756 (263 residues), 43.4 bits, see alignment E=3.4e-15 TIGR00461: glycine dehydrogenase" amino acids 29 to 971 (943 residues), 1476 bits, see alignment E=0 PF00266: Aminotran_5" amino acids 599 to 726 (128 residues), 23.3 bits, see alignment E=4.5e-09 PF21478: GcvP2_C" amino acids 799 to 920 (122 residues), 201 bits, see alignment E=6.8e-64

Best Hits

Swiss-Prot: 60% identical to GCSP2_COLP3: Glycine dehydrogenase (decarboxylating) 2 (gcvP2) from Colwellia psychrerythraea (strain 34H / ATCC BAA-681)

Predicted SEED Role

"Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2)" in subsystem Glycine and Serine Utilization or Glycine cleavage system or Photorespiration (oxidative C2 cycle) (EC 1.4.4.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T7I8 at UniProt or InterPro

Protein Sequence (978 amino acids)

>BPHYT_RS19330 glycine cleavage system P protein (EC 1.4.4.2) (Burkholderia phytofirmans PsJN)
MKLEHPDRLMNRTPLSLAALEVHDAFAERHIGPDAADQHAMLEALGFASRAALIDAVIPK
TIRRTETLPLGPFTQPKSEAEALAALRELADKNQVFRSYIGQGYYNAHTPTVILRNVLEN
PAWYTAYTPYQPEISQGRLEALLNFQQMIVDLTGLAISNASLLDEATAAAEAMTLLQRVG
KPKSNVFFVADDVLPQTIEVVRTRATPVGIEVKVGPASEAANANAFGVLLQYPGVNGDVR
DYRALTEAIHAAGGHVVVAADLLALTVLTPPGEWGADVAVGNTQRFGVPVGFGGPHAAYL
AVRDEFKRQMPGRLVGVTVDAQGNPALRLALQTREQHIRREKATSNVCTAQALLAIMASM
YAVYHGPHGLKTIALRVNRIAALLAEGAKQLGYTLANETFFDTLTFDTGARTQALLDAAT
AKRINLRRVSATQVGLSIDETTTRHDLADLLAVFAQAAFTNDVPQVDALDAKLAASNTAS
VPAALERTSAYLTHHVFNRHHSETEMLRYLRSLSDKDLALDRSMIPLGSCTMKLNATSEM
LPVTWPEFGQIHPFAPAEQTVGYREMIDQLEEMLVAATGYAAVSLQPNAGSQGEYAGLLI
IHAYHASRGEAHRNVCLIPASAHGTNPASAQMAGMQVVVVACDAQGNVDIEDLKKKAGQH
ADKLAAIMITYPSTHGVFEQNVREICEIVHAHGGQVYVDGANMNAMVGLTAPGQFGGDVS
HLNLHKTFCIPHGGGGPGVGPVAVGAHLAQFLPNQISSGYERAPNGIGAVSGAPYGSASI
LPISWMYIAMMGAKNLTAATETAILNANYVAKKLAPHYPVLYSGPGGLVAHECILDLRPI
KETSGITVDDVAKRLADYGFHAPTMSFPVPGTLMVEPTESESKEELDRFIEAMIAIREEI
RAVEDGRSDREDNPLKHAPHTAAVVIANDWKHAYARETAAYPLPTLIAKKYWPPVGRADN
VYGDRNLFCSCVPIADYE