Protein Info for BPHYT_RS18155 in Burkholderia phytofirmans PsJN

Annotation: DNA-directed RNA polymerase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1368 signal peptide" amino acids 1 to 40 (40 residues), see Phobius details TIGR02013: DNA-directed RNA polymerase, beta subunit" amino acids 8 to 1364 (1357 residues), 1822.7 bits, see alignment E=0 PF04563: RNA_pol_Rpb2_1" amino acids 27 to 506 (480 residues), 86.5 bits, see alignment E=5.8e-28 PF04561: RNA_pol_Rpb2_2" amino acids 156 to 228 (73 residues), 28.4 bits, see alignment 3.9e-10 amino acids 361 to 460 (100 residues), 53 bits, see alignment 1.1e-17 PF04565: RNA_pol_Rpb2_3" amino acids 519 to 586 (68 residues), 107.3 bits, see alignment 1e-34 PF10385: RNA_pol_Rpb2_45" amino acids 597 to 662 (66 residues), 89.3 bits, see alignment 4e-29 PF00562: RNA_pol_Rpb2_6" amino acids 726 to 1290 (565 residues), 450.3 bits, see alignment E=1.8e-138 PF04560: RNA_pol_Rpb2_7" amino acids 1292 to 1365 (74 residues), 93.1 bits, see alignment 3.5e-30

Best Hits

KEGG orthology group: K03043, DNA-directed RNA polymerase subunit beta [EC: 2.7.7.6] (inferred from 78% identity to aav:Aave_4531)

Predicted SEED Role

"DNA-directed RNA polymerase beta subunit (EC 2.7.7.6)" in subsystem RNA polymerase bacterial (EC 2.7.7.6)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.6

Use Curated BLAST to search for 2.7.7.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T759 at UniProt or InterPro

Protein Sequence (1368 amino acids)

>BPHYT_RS18155 DNA-directed RNA polymerase subunit beta (Burkholderia phytofirmans PsJN)
MQYSFTEKKRIRKSFAKRPIVHQVPFLLATQLESFSTFLQADTSSTQRKPEGLQAAFTSV
FPIVSHNGFARLEFVSYMLSPPAFNIKECQQRGLTYCSALRAKVRLVLLDKESPSKPVVK
EVKEQEVYMGEIPLMTPTGSFVINGTERVIVSQLHRSPGVFFEHDKGKTHSSGKLLFSAR
IIPYRGSWLDFEFDPKDVLYFRVDRRRKMPVTILLKAIGLTPEQILANFFVFDNFTLMPE
GAQMEFVPERLRGEVARFDITDRDGNVIVQKDKRINAKHIRDLDNAKTKFISVPEDYLLG
RVLAKNVVDGDTGEVIANANDEITETVLEKLRESKIKDIQTLYTNDLDQGPYISSTLRID
ETADKMAARIAIYRMMRPGEPPTEEAVEALFNRLFYSEDAYDLSKVGRMKFNRRVGRDEI
VGPMTLQDDDILATIKILVELRNGKGEVDDIDHLGNRRVRCVGELAENQFRAGLVRVERA
VKERLGQAESENLMPHDLINSKPISSAIREFFGSSQLSQFMDQTNPLSEITHKRRVSALG
PGGLTRERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLALYAHLNEYGFLETPYRKV
VDSKVTDQIDYLSAIEEGRYVIAQANAAVAEDGSLTDELVSSREAGETLMVTPDRIQYMD
VAPSQIVSVAASLIPFLEHDDANRALMGSNMQRQAVPCLRPEKAVVGTGIERTVAVDSGT
TVQAFRGGVVDYVDAGRMVIRVNDDEAAAGETGVDIYNLIKYTRSNQNTNINQRPIVKVG
DIVSRGDVLADGASTDLGELALGQNMLVAFMPWNGYNFEDSILISEKVVADDRYTSIHIE
ELNVVARDTKLGPEEITRDISNLAEVQLGRLDESGIVYIGAEVEAGDVLVGKVTPKGETQ
LTPEEKLLRAIFGEKASDVKDTSLRVPSGMSGTVIDVQVFTREGIQRDKRAQQIIDDELK
RYRLDLNDQLRIVEGDAFQRLARMLDGKVANGGPKKLAKGTKIEQAYLQDLDHYHWFDIR
LADEEAAAQLEAIKDSIEQKRHQFDLAFEEKRKKLTQGDELPPGVLKMVKVYLAVKRRLQ
PGDKMAGRHGNKGVVSKIVPIEDMPYMADGRPADVVLNPLGVPSRMNVGQVLEVHLGWAA
KGLGWRIGEMLQRQAKIAELREFLTKIYNESGRAEELDSFTDDEIVELAKNLREGVPFAT
PVFDGATEEEMSRALDLAFPDDIAKNLGMTPSKNQVRLYDGRTGEMFERTVTVGYMHYLK
LHHLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASYVLQEMLTVK
SDDVTGRTKVYENLVKGDHVIDAGMPESFNVLVKEIRSLGIDIDLDRN