Protein Info for BPHYT_RS17540 in Burkholderia phytofirmans PsJN

Updated annotation (from data): L-aspartate/L-glutamate/L-glutamine:H+ symporter
Rationale: Specifically important for utilization of glutamine, glutamate, or asparagine.
Original annotation: amino acid:proton symporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 531 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 38 to 61 (24 residues), see Phobius details amino acids 82 to 103 (22 residues), see Phobius details amino acids 130 to 151 (22 residues), see Phobius details amino acids 163 to 182 (20 residues), see Phobius details amino acids 195 to 215 (21 residues), see Phobius details amino acids 235 to 258 (24 residues), see Phobius details amino acids 278 to 300 (23 residues), see Phobius details amino acids 342 to 359 (18 residues), see Phobius details amino acids 364 to 386 (23 residues), see Phobius details amino acids 399 to 420 (22 residues), see Phobius details amino acids 426 to 443 (18 residues), see Phobius details amino acids 457 to 475 (19 residues), see Phobius details amino acids 487 to 505 (19 residues), see Phobius details PF13520: AA_permease_2" amino acids 10 to 423 (414 residues), 179.9 bits, see alignment E=8.7e-57 PF00324: AA_permease" amino acids 15 to 467 (453 residues), 114.4 bits, see alignment E=5.7e-37

Best Hits

Swiss-Prot: 63% identical to YBEC_BACSU: Probable amino acid-proton symporter YbeC (ybeC) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_3527)

Predicted SEED Role

"Amino acid transporter"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SZ32 at UniProt or InterPro

Protein Sequence (531 amino acids)

>BPHYT_RS17540 L-aspartate/L-glutamate/L-glutamine:H+ symporter (Burkholderia phytofirmans PsJN)
MKSSIQRNIGPFALMLTGLGSIIGSGWLFGAWKAAKIAGPAAVCAWVIGAVVILAIALTY
AELGAMFPESGGMVRYARYSHGALVGFISAWANWIAIVSVIPIEAEASIQYMSTWPYPWA
HALFVDGSLTTNGLLLSAALVIIYFMLNYWGVKLFARANSAITIFKFLIPGATILGLMFA
GFHKENFGEASTFAPYGWSAVLTAVSTSGIVFAFNGFQSPINLAGEARNPAKSVPFAVIG
SILLALVIYVLLQIAYIGAVNPSDVMKGWSHFNFASPFAELAIALNLNWLAILLYVDAFV
SPSGTGTTYMATTSRMIYAMERNNTMPKMFGNVHPFYGVPRQAMWFNLLVSFIFLFFFRG
WSSLAAVISVATVISYLTGPISLMALRRAATDLERPLHIPGMKIIAPFAFVCASLILYWA
KWPLTGEIILLMVVALPVYFYFQAKSGFAGWGRDLKAAWWLVAYLPVMAILSLIGSKQFG
GHDLIPYGWDMVVVIAFSLVFYYWGVTSGYRSEYLDERSEHDEVLEGMGAH