Protein Info for BPHYT_RS17380 in Burkholderia phytofirmans PsJN

Annotation: ribulose-phosphate 3-epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 227 TIGR01163: ribulose-phosphate 3-epimerase" amino acids 6 to 219 (214 residues), 315.5 bits, see alignment E=6.7e-99 PF00834: Ribul_P_3_epim" amino acids 6 to 206 (201 residues), 296.9 bits, see alignment E=5.8e-93

Best Hits

Swiss-Prot: 70% identical to RPE_ECOL6: Ribulose-phosphate 3-epimerase (rpe) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K01783, ribulose-phosphate 3-epimerase [EC: 5.1.3.1] (inferred from 100% identity to bpy:Bphyt_3494)

MetaCyc: 70% identical to ribulose-phosphate 3-epimerase (Escherichia coli K-12 substr. MG1655)
Ribulose-phosphate 3-epimerase. [EC: 5.1.3.1]

Predicted SEED Role

"Ribulose-phosphate 3-epimerase (EC 5.1.3.1)" in subsystem Calvin-Benson cycle or Conserved gene cluster associated with Met-tRNA formyltransferase or Pentose phosphate pathway or Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization (EC 5.1.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SYZ9 at UniProt or InterPro

Protein Sequence (227 amino acids)

>BPHYT_RS17380 ribulose-phosphate 3-epimerase (Burkholderia phytofirmans PsJN)
MTQFRIAPSILSADFARLGEEVRNVVAAGADWIHFDVMDNHYVPNLTIGPLVCEAIRPHV
DVPIDVHLMVRPVDRIVPDFAKAGANLISFHPEGSDHIDRTLSLIRDHGCKAGLVFNPAT
SLSHLDYVMDKVDLVLIMSVNPGFGGQSFIPEALVKLREARKRINEYKERTGREIHLEVD
GGVKVDNIAEIAAAGADTFVAGSAIFGQPDHKVVIEKMRAALANIQH