Protein Info for BPHYT_RS17345 in Burkholderia phytofirmans PsJN

Updated annotation (from data): bifunctional thiolase PaaJ: 3-oxo-5,6-didehydrosuberyl-CoA thiolase (EC 2.3.1.223); 3-oxoadipyl-CoA thiolase (EC 2.3.1.174)
Rationale: Specifically important for utilization of phenylacetate and phenylalanine. This is probably the bifunctional thiolase in the aerobic phenylacetyl-CoA degradation pathway (also known as PaaJ).
Original annotation: acetyl-CoA acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 transmembrane" amino acids 379 to 397 (19 residues), see Phobius details TIGR02430: 3-oxoadipyl-CoA thiolase" amino acids 3 to 400 (398 residues), 718.7 bits, see alignment E=2e-220 PF00108: Thiolase_N" amino acids 5 to 267 (263 residues), 242.5 bits, see alignment E=7.6e-76 TIGR01930: acetyl-CoA C-acyltransferase" amino acids 6 to 398 (393 residues), 438.9 bits, see alignment E=1.6e-135 PF00109: ketoacyl-synt" amino acids 80 to 123 (44 residues), 23.1 bits, see alignment 7.7e-09 PF02803: Thiolase_C" amino acids 277 to 399 (123 residues), 151.2 bits, see alignment E=1.5e-48

Best Hits

Swiss-Prot: 69% identical to CATF_ACIAD: Beta-ketoadipyl-CoA thiolase (catF) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)

KEGG orthology group: K00632, acetyl-CoA acyltransferase [EC: 2.3.1.16] (inferred from 100% identity to bpy:Bphyt_3487)

MetaCyc: 79% identical to 3-oxoadipyl-CoA thiolase (Cupriavidus necator H16)
3-oxoadipyl-CoA thiolase. [EC: 2.3.1.174]

Predicted SEED Role

"Acetyl-CoA acetyltransferase (EC 2.3.1.9) @ Beta-ketoadipyl CoA thiolase (EC 2.3.1.-)" (EC 2.3.1.-, EC 2.3.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.-, 2.3.1.16, 2.3.1.174, 2.3.1.9

Use Curated BLAST to search for 2.3.1.- or 2.3.1.16 or 2.3.1.174 or 2.3.1.223 or 2.3.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SYZ2 at UniProt or InterPro

Protein Sequence (400 amino acids)

>BPHYT_RS17345 bifunctional thiolase PaaJ: 3-oxo-5,6-didehydrosuberyl-CoA thiolase (EC 2.3.1.223); 3-oxoadipyl-CoA thiolase (EC 2.3.1.174) (Burkholderia phytofirmans PsJN)
MNDAFICDAIRTPIGRYGGALKDVRADDLGAVPIKALIERNPGVDWRTVDDVIYGCANQA
GEDNRNVARMSALLAGLPVEAPGSTINRLCGSGMDAVGTAARAIKAGEARLMIAGGVESM
TRAPFVMGKATSAFARQADIYDTTIGWRFINPLMKRQYGVDSMPETAENVAAEFSVSRAD
QDAFALASQQKAARAQQDGTLAQEIVGVEIAQKKGDPVRALLDEHPRETSLESLGKLKGV
VRPDGSVTAGNASGVNDGACALLLANQQAADQYGLRRRARVVGMATAGVEPRIMGIGPAP
ATQKLLKQLGMTLEQLDVIELNEAFASQGLAVLRTLGLRDDDPRVNPNGGAIALGHPLGA
SGARLITTALYQLERTNGRFALCTMCIGVGQGIALVIERL