Protein Info for BPHYT_RS17095 in Burkholderia phytofirmans PsJN

Annotation: proline--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00409: proline--tRNA ligase
  • amino acids 1 to 566 (566 residues), 766.7 bits, E=9e-235
• PF00587: tRNA-synt_2b
  • amino acids 95 to 461 (367 residues), 113.7 bits, E=1.6e-36
• PF04073: tRNA_edit
  • amino acids 257 to 380 (124 residues), 100.6 bits, E=9.8e-33
• PF03129: HGTP_anticodon
  • amino acids 478 to 568 (91 residues), 65.3 bits, E=6.8e-22

Best Hits

Swiss-Prot: 100% identical to SYP_PARPJ: Proline--tRNA ligase (proS) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K01881, prolyl-tRNA synthetase [EC: 6.1.1.15] (inferred from 100% identity to bpy:Bphyt_3443)

MetaCyc: 57% identical to proline--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Cysteine--tRNA ligase. [EC: 6.1.1.16]; Proline--tRNA ligase. [EC: 6.1.1.16, 6.1.1.15]; 6.1.1.15 [EC: 6.1.1.16, 6.1.1.15]; 3.1.1.M26 [EC: 6.1.1.16, 6.1.1.15, 3.1.1.M26]

Predicted SEED Role

"Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type" (EC 6.1.1.15)

MetaCyc Pathways

• tRNA charging (20/21 steps found)
• pyoluteorin biosynthesis (1/7 steps found)
• thiocoraline biosynthesis (1/7 steps found)
• ergotamine biosynthesis (4/12 steps found)
• anguibactin biosynthesis (1/8 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• clorobiocin biosynthesis (1/10 steps found)
• pyochelin biosynthesis (1/11 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• pyrrolomycin biosynthesis (1/14 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (6/25 steps found)
• phosphinothricin tripeptide biosynthesis (6/25 steps found)
• colibactin biosynthesis (6/38 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Cysteine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.15, 6.1.1.16

Use Curated BLAST to search for 3.1.1.M26 or 6.1.1.15 or 6.1.1.16

Sequence Analysis Tools

See B2SYU8 at UniProt or InterPro

Protein Sequence (578 amino acids)

>BPHYT_RS17095 proline--tRNA ligase (Burkholderia phytofirmans PsJN)
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPVGLRSIRKVEAIVREE
MNRAGGIELLMPAVQPAELWQESGRWEKYGPELLRFKDRRQSDFVIGPTHEEVVTDIARN
QIKSYRQLPVNFYQIQTKFRDEIRPRFGVMRGREFIMKDAYSFDKDMDGLRESYRKMYDA
YVRIFTRLGLDFRAVAADNGSIGGSGSHEFHVIAETGEDAIAYCPTSDFAANVEAAEALP
LYAERAAPTEEMKKTPTPGKAKCEAVAELLNIPLERTIKSIILATENEGAEPTIWLLMLR
GDHDLNEIKASKLPGLAEFRMATEAEIIETFGTPPGYLGPIGTKKPIKVVADRTVANMSD
FVVGSNEVDYHTTGVNWGRDLPEPVVADIRNVKKGDPSPDGKGVLDICRGIEVGHVFQLG
TKYSEAMGATCLDETGKPRPMEMGCYGIGVTRILGAAIEQNFDDKGIIWPESIAPFEVVL
CPMGYDRSEAVRAEADKLYAALVEAGIDVILDDRGERPGVMFADWELIGVPHRLVIGDRG
LKEGKLEYQGRRDAEATLLPVEDAAQTVIGKVRAALAR