Protein Info for BPHYT_RS16940 in Burkholderia phytofirmans PsJN

Annotation: short-chain dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00106: adh_short
  • amino acids 7 to 200 (194 residues), 201.3 bits, E=1.7e-63
• PF08659: KR
  • amino acids 9 to 121 (113 residues), 44.9 bits, E=1.9e-15
• PF13561: adh_short_C2
  • amino acids 13 to 251 (239 residues), 223.6 bits, E=4.3e-70

Best Hits

Swiss-Prot: 40% identical to 3BHD1_EGGLE: 3-beta-hydroxycholanate 3-dehydrogenase (NAD(+)) 1 (Elen_0198) from Eggerthella lenta (strain ATCC 25559 / DSM 2243 / JCM 9979 / NCTC 11813 / VPI 0255)

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_3412)

MetaCyc: 40% identical to NAD-dependent bile acid 3beta-dehydrogenase (Eggerthella lenta DSM 2243)
RXN-11472 [EC: 1.1.1.391]; 1.1.1.391 [EC: 1.1.1.391]; 1.1.1.391 [EC: 1.1.1.391]; 1.1.1.391 [EC: 1.1.1.391]

Predicted SEED Role

"3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100)" in subsystem Fatty Acid Biosynthesis FASII or mycolic acid synthesis (EC 1.1.1.100)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (50/53 steps found)
• palmitate biosynthesis III (28/29 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• anteiso-branched-chain fatty acid biosynthesis (30/34 steps found)
• even iso-branched-chain fatty acid biosynthesis (30/34 steps found)
• odd iso-branched-chain fatty acid biosynthesis (30/34 steps found)
• tetradecanoate biosynthesis (mitochondria) (23/25 steps found)
• superpathway of unsaturated fatty acids biosynthesis (E. coli) (19/20 steps found)
• octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) (12/12 steps found)
• oleate biosynthesis IV (anaerobic) (13/14 steps found)
• palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (9/9 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (14/16 steps found)
• biotin biosynthesis I (13/15 steps found)
• cis-vaccenate biosynthesis (5/5 steps found)
• fatty acid elongation -- saturated (5/5 steps found)
• gondoate biosynthesis (anaerobic) (4/4 steps found)
• 8-amino-7-oxononanoate biosynthesis I (9/11 steps found)
• (5Z)-dodecenoate biosynthesis I (5/6 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• 8-amino-7-oxononanoate biosynthesis IV (4/5 steps found)
• (5Z)-dodecenoate biosynthesis II (4/6 steps found)
• stearate biosynthesis IV (4/6 steps found)
• iso-bile acids biosynthesis I (2/4 steps found)
• bile acids 3-O-epimerization (1/4 steps found)
• petroselinate biosynthesis (2/6 steps found)
• 2-allylmalonyl-CoA biosynthesis (2/8 steps found)
• streptorubin B biosynthesis (20/34 steps found)
• bile acids epimerization (1/12 steps found)
• mycolate biosynthesis (25/205 steps found)
• superpathway of mycolate biosynthesis (26/239 steps found)

KEGG Metabolic Maps

• Biosynthesis of unsaturated fatty acids
• Fatty acid biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.100

Use Curated BLAST to search for 1.1.1.100 or 1.1.1.391

Sequence Analysis Tools

See B2SYR7 at UniProt or InterPro

Protein Sequence (258 amino acids)

>BPHYT_RS16940 short-chain dehydrogenase (Burkholderia phytofirmans PsJN)
MKRLAGKVALVTGAGRGIGSAIAHAFAREGAAVVLAELDIETARQTAEHIKAQTGARVLA
VHTDVTQSASVQHAVSEAERAFGALDVLVNNAGINVFCDPLTMTDDDWRRCFAVDLDGVW
NGCRAALPGMVERGAGSIVNIASTHSFKIIPGCFPYPVAKHGVIGLTRALGIEYAPRNVR
VNAIAPGYIETQLTHDWWNEQADPAAAQQATLDLQPMKRIGRPEEVAMTAVFLASDEAPF
INATCITVDGGRSALYHD