Protein Info for BPHYT_RS16735 in Burkholderia phytofirmans PsJN

Annotation: phosphogluconate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 634 TIGR01196: phosphogluconate dehydratase" amino acids 5 to 605 (601 residues), 982.5 bits, see alignment E=3.4e-300 PF00920: ILVD_EDD" amino acids 69 to 602 (534 residues), 545.5 bits, see alignment E=6.5e-168

Best Hits

Swiss-Prot: 63% identical to EDD_PSEAE: Phosphogluconate dehydratase (edd) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01690, phosphogluconate dehydratase [EC: 4.2.1.12] (inferred from 100% identity to bpy:Bphyt_3370)

MetaCyc: 60% identical to phosphogluconate dehydratase (Escherichia coli K-12 substr. MG1655)
Phosphogluconate dehydratase. [EC: 4.2.1.12]

Predicted SEED Role

"Phosphogluconate dehydratase (EC 4.2.1.12)" in subsystem Entner-Doudoroff Pathway (EC 4.2.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SYM6 at UniProt or InterPro

Protein Sequence (634 amino acids)

>BPHYT_RS16735 phosphogluconate dehydratase (Burkholderia phytofirmans PsJN)
MVSPHSQLLKVTQRVVERSKPTREAYLARIHQAQGKFPARGALSCANLAHGFAGLEGNDK
LVIKQIREPNIGIVSSYNEMLSAHAPYKNYPDIIKQAARENGGVAQFAGGVPAMCDGVTQ
GNAGMELSLFSREVIAMSTAVALTHNMFDAALCLGICDKIVPGLLIGALQFGHIPTIFVP
AGPMGSGLSNDDKAKTRQLFATGQCDRGALLEAEAAAYHSHGTCTFYGTANSNQMLMEVM
GLHLPSSAFVHPHTPLRDALTAQAARRVLELTVERGNYTPIGHVVDEKAIVNGIVALLAT
GGSTNHTLHLVAIARAAGIVIDWDDFDTLSQAVPLLAKIYPNGKADVNHFHAAGGVAFLV
RNLLEGGLLHEDVNTVAGKGLKHYTEEPKLIDGKLQWVPGAQASEDTAVLRGIKEPFQPD
GGLRLMQGKLGRGVIKISAVAAQHRKVKAPAIVFDSQEAVQEAFDKGELKRDFIAVVRFQ
GARANGMPELHRLTPLLGVLQDQGFHVALVTDGRMSGASGKVPAVIHLSPEALLQGPIGK
VRTGDMLVIDAEAGVLDIEIDAAEWAARPNAVPQHQAENEVGFGRELFSVFRAAAAPAEQ
GASVFGAMVGERSAHHGEAAKAHTAKHTSTTHAS