Protein Info for BPHYT_RS16635 in Burkholderia phytofirmans PsJN

Annotation: D-aminoacyl-tRNA deacylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 152 TIGR00256: D-tyrosyl-tRNA(Tyr) deacylase" amino acids 1 to 150 (150 residues), 174 bits, see alignment E=1.4e-55 PF02580: Tyr_Deacylase" amino acids 3 to 149 (147 residues), 170.1 bits, see alignment E=1.9e-54

Best Hits

Swiss-Prot: 100% identical to DTD_PARPJ: D-aminoacyl-tRNA deacylase (dtd) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K07560, D-tyrosyl-tRNA(Tyr) deacylase [EC: 3.1.-.-] (inferred from 98% identity to bxe:Bxe_A0612)

MetaCyc: 60% identical to D-aminoacyl-tRNA deacylase (Escherichia coli K-12 substr. MG1655)
RXN-15041 [EC: 3.1.1.96]; 3.1.1.96 [EC: 3.1.1.96]; 3.1.1.96 [EC: 3.1.1.96]; 3.1.1.96 [EC: 3.1.1.96]; 3.1.1.96 [EC: 3.1.1.96]

Predicted SEED Role

"D-tyrosyl-tRNA(Tyr) deacylase (EC 3.6.1.n1)" (EC 3.6.1.n1)

Isozymes

Compare fitness of predicted isozymes for: 3.1.-.-

Use Curated BLAST to search for 3.1.-.- or 3.1.1.96 or 3.6.1.n1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SYK5 at UniProt or InterPro

Protein Sequence (152 amino acids)

>BPHYT_RS16635 D-aminoacyl-tRNA deacylase (Burkholderia phytofirmans PsJN)
MIALIQRVRRAEVRVADRVTGAIEAGLLALVCAERGDSEAAADRLLAKVLGYRVFSDAAG
KMNLSVQNLDGAGRAGGLLLVSQFTLAADTNSGLRPSFTPAAPPDEGKRLFDYFVAAARA
KHPIVETGEFGADMQVSLVNDGPVTFWLQTNA