Protein Info for BPHYT_RS16535 in Burkholderia phytofirmans PsJN

Annotation: tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 457 TIGR01574: tRNA-i(6)A37 thiotransferase enzyme MiaB" amino acids 4 to 446 (443 residues), 582.4 bits, see alignment E=6.6e-179 PF00919: UPF0004" amino acids 4 to 104 (101 residues), 102.7 bits, see alignment E=1.4e-33 TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family" amino acids 4 to 443 (440 residues), 489.2 bits, see alignment E=1e-150 PF04055: Radical_SAM" amino acids 151 to 326 (176 residues), 96.9 bits, see alignment E=2.3e-31 PF01938: TRAM" amino acids 381 to 446 (66 residues), 55.1 bits, see alignment E=8.4e-19

Best Hits

Swiss-Prot: 100% identical to MIAB_PARPJ: tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase (miaB) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K06168, bifunctional enzyme involved in thiolation and methylation of tRNA (inferred from 100% identity to bpy:Bphyt_3329)

MetaCyc: 64% identical to isopentenyl-adenosine A37 tRNA methylthiolase (Escherichia coli K-12 substr. MG1655)
RXN0-5063 [EC: 2.8.4.3]

Predicted SEED Role

"tRNA-i(6)A37 methylthiotransferase" in subsystem Ribosomal protein S12p Asp methylthiotransferase or tRNA processing

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.4.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SYI5 at UniProt or InterPro

Protein Sequence (457 amino acids)

>BPHYT_RS16535 tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase (Burkholderia phytofirmans PsJN)
MTKKVYVKTFGCQMNEYDSDKMVDVLGAAEGLVKTDTPEDADVILFNTCSVREKAQEKVF
SDLGRVRELKEANPNLIIGVGGCVASQEGAAIVSRAPYVDLVFGPQTLHRLPQMIDKRRE
SGRAQVDISFPEIEKFDHLPPARVDGPSAFVSIMEGCSKYCSYCVVPYTRGEEVSRPLDD
VLTEIAGLADQGVREVTLLGQNVNAYRAGLTLGSTEIADFAQLIEYVADIPGIERIRYTT
SHPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP
DLSLSTDMIVGFPGETEEDFDKMMALVHEMKYDTSFSFIYSPRPGTPAANLHDDTPREVK
LKRLQHLQATIEENVQRISDSMVGKIERILVERPARKDPNELAGRTENNRVVNFPAPIAS
HARLIGQMVDVKIVKAYPHSLRGELVLVHDDAPATTH