Protein Info for BPHYT_RS16490 in Burkholderia phytofirmans PsJN
Annotation: glycerol-3-phosphate dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 57% identical to GLPD_PSEAE: Glycerol-3-phosphate dehydrogenase (glpD) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K00111, glycerol-3-phosphate dehydrogenase [EC: 1.1.5.3] (inferred from 100% identity to bpy:Bphyt_3320)MetaCyc: 53% identical to D-erythritol 1-phosphate dehydrogenase (Brucella abortus)
RXN-17768 [EC: 1.1.1.402]
Predicted SEED Role
"Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or Respiratory dehydrogenases 1 (EC 1.1.5.3)
MetaCyc Pathways
- glycerol-3-phosphate shuttle (2/2 steps found)
- glycerol-3-phosphate to cytochrome bo oxidase electron transfer (2/2 steps found)
- glycerophosphodiester degradation (2/2 steps found)
- glycerol and glycerophosphodiester degradation (3/4 steps found)
- glycerol degradation I (2/3 steps found)
- glycerol 3-phosphate to cytochrome aa3 oxidase electron transfer (1/2 steps found)
- glycerol-3-phosphate to fumarate electron transfer (1/2 steps found)
- glycerol-3-phosphate to hydrogen peroxide electron transport (1/2 steps found)
- nitrate reduction IX (dissimilatory) (1/2 steps found)
- sn-glycerol 3-phosphate anaerobic respiration (1/3 steps found)
- erythritol degradation I (1/6 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.1.1.402 or 1.1.5.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See B2SYH6 at UniProt or InterPro
Protein Sequence (509 amino acids)
>BPHYT_RS16490 glycerol-3-phosphate dehydrogenase (Burkholderia phytofirmans PsJN) MTQGSRYDLLVVGGGINGAGIARDAAGRGLSVLLCEQDDLAAHTSSASTKLIHGGLRYLE YREFGLVRKALQERETLLRAAPHIMWPLRFVMPHMPDLRPAWLIRAGLFLYDHLARRELL PGSRGIVMRNHPAGAPLVDSIKRGFVYSDGWVNDARLVVLNALDAQEHGAKILTRTKLLS AVRAGGEWRAQLKRADGTLLDVRAASIANAAGPWVGELLQGALGRAASHSVRLVKGSHIV TRRLFEHDHAYIFQNPDKRIIFAIPYEHDYTLIGTTDLEYRGDPSQVAINADETQYLCDS INRYFKQKISPADVRWTYSGVRPLLEEEGADNPSAVTRDYSLELDAPAGEAPLLSVFGGK ITTFRKLAEEAVDKLAQALQNGASSWTAGAPLPGGDIPNANFERFLSEFKQQHAWLPADL AHRLARAYGTRVKQVLGNARSVADLGRAFAPGLYEAELTYLRDTEWARSAQDVLWRRSKL GLHVEPGTLDSITRDIDAWFAREPAREGA