Protein Info for BPHYT_RS16405 in Burkholderia phytofirmans PsJN
Updated annotation (from data): galactonate dehydratase [EC: 4.2.1.6]
Rationale: Specifically important for: D-Galactose. galactonate is an intermediate in galactose oxidation (KEGG_correct)
Original annotation: galactonate dehydratase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 65% identical to DGOD_ECOUT: D-galactonate dehydratase (dgoD) from Escherichia coli (strain UTI89 / UPEC)
KEGG orthology group: K01684, galactonate dehydratase [EC: 4.2.1.6] (inferred from 100% identity to bpy:Bphyt_3303)MetaCyc: 64% identical to D-galactonate dehydratase (Escherichia coli K-12 substr. MG1655)
Galactonate dehydratase. [EC: 4.2.1.140, 4.2.1.6]
Predicted SEED Role
"Gluconate dehydratase (EC 4.2.1.39)" in subsystem Entner-Doudoroff Pathway (EC 4.2.1.39)
MetaCyc Pathways
- Entner-Doudoroff pathway III (semi-phosphorylative) (8/9 steps found)
- D-galactonate degradation (3/3 steps found)
- Entner-Doudoroff pathway II (non-phosphorylative) (6/9 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.2.1.39
Use Curated BLAST to search for 4.2.1.140 or 4.2.1.39 or 4.2.1.6
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See B2SYF9 at UniProt or InterPro
Protein Sequence (382 amino acids)
>BPHYT_RS16405 galactonate dehydratase [EC: 4.2.1.6] (Burkholderia phytofirmans PsJN) MKITKLETFIVPPRWCFLKIETDEGIVGWGEPVVEGRAHTVAAAVEELSDYLIGKDPLLI EDHWQVMYRSGFYRGGPITMSAIAGVDQALWDIKGKHHGVPIHALLGGQVRDKIKVYSWI GGDRPSDVANNARAVVERGFKAVKMNGSEELQIIDTFDKVQGVINNVAAVREAVGPNIGI GVDFHGRVHKPMAKVLAKELDPYKLLFIEEPVLSENAEALRDIVNQTNTPIALGERLYSR WDFKHILSGGYVDIIQPDASHAGGITECRKIASMAEAYDVALALHCPLGPIALATCLQID AVSYNAFIQEQSLGIHYNQGNDLLDYIKNPEVFKYEDGFVSIPQGPGLGIEVNEEKVREM AKVGHRWRNPVWRHEDGSVAEW