Protein Info for BPHYT_RS16175 in Burkholderia phytofirmans PsJN

Annotation: hemolysin D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 410 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF00529: CusB_dom_1" amino acids 35 to 359 (325 residues), 39.3 bits, see alignment E=1e-13 TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 37 to 373 (337 residues), 261.4 bits, see alignment E=5e-82 PF16576: HlyD_D23" amino acids 62 to 291 (230 residues), 42.3 bits, see alignment E=1.1e-14 PF13533: Biotin_lipoyl_2" amino acids 63 to 110 (48 residues), 25.5 bits, see alignment 1.7e-09

Best Hits

Swiss-Prot: 51% identical to MEPA_PSEPU: Multidrug/solvent efflux pump periplasmic linker protein MepA (mepA) from Pseudomonas putida

KEGG orthology group: K03585, membrane fusion protein (inferred from 100% identity to bpy:Bphyt_3258)

MetaCyc: 51% identical to multidrug efflux pump membrane fusion lipoprotein AcrA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-1551; TRANS-RXN-1552; TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN-360; TRANS-RXN0-592

Predicted SEED Role

"Membrane fusion protein of RND family multidrug efflux pump" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SYB4 at UniProt or InterPro

Protein Sequence (410 amino acids)

>BPHYT_RS16175 hemolysin D (Burkholderia phytofirmans PsJN)
MRVERVPFRLISAATAAVLLAACGPKQSAPPQQTPEVGVVTVQPTAVPVVTELPGRTSAF
LVAQVRARVDGIVLRREFTEGSQVKAGQRLYKIDPAPYIATLNNAKATLAKAQANLVSTT
AQANRYKVLVAANAVSKQDYDNAVASEGQAAADVAAGKAAVDTAQINLGYTDVVSPVSGQ
VGISQVTPGAYVQASAATLMATVQTLDPVYVDLTQSSLDGLKLRRDMQEGRLKTTGPDAA
KVSLILEDGRTYSEKGKLQFTDVTVDQGTGSVTVRAIFKNADKVLLPGMFVRARIEEGVN
ENALVVPQVGITHDQKGQPTALVVGDDNKVALRQLVTSGTYGSNWVVESGLKPGDRVIVQ
GTDKAKPGMQVKTVAAQLPATPASGAAAQSAPAASGAAQTAQPASAASGA