Protein Info for BPHYT_RS16170 in Burkholderia phytofirmans PsJN

Annotation: multidrug efflux RND transporter permease subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1073 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 340 to 359 (20 residues), see Phobius details amino acids 366 to 389 (24 residues), see Phobius details amino acids 395 to 415 (21 residues), see Phobius details amino acids 438 to 458 (21 residues), see Phobius details amino acids 470 to 497 (28 residues), see Phobius details amino acids 540 to 558 (19 residues), see Phobius details amino acids 873 to 891 (19 residues), see Phobius details amino acids 898 to 918 (21 residues), see Phobius details amino acids 924 to 948 (25 residues), see Phobius details amino acids 973 to 991 (19 residues), see Phobius details amino acids 1002 to 1027 (26 residues), see Phobius details TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 1 to 1045 (1045 residues), 1714.7 bits, see alignment E=0 PF00873: ACR_tran" amino acids 1 to 1029 (1029 residues), 1321.4 bits, see alignment E=0 PF03176: MMPL" amino acids 338 to 514 (177 residues), 26.5 bits, see alignment E=3.1e-10

Best Hits

Swiss-Prot: 66% identical to ACRB_ECOLI: Multidrug efflux pump subunit AcrB (acrB) from Escherichia coli (strain K12)

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 67% identity to abo:ABO_0964)

MetaCyc: 66% identical to multidrug efflux pump RND permease AcrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN0-592

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SYB3 at UniProt or InterPro

Protein Sequence (1073 amino acids)

>BPHYT_RS16170 multidrug efflux RND transporter permease subunit (Burkholderia phytofirmans PsJN)
MAKFFIDRPIFAWVIAIILMLAGIASVFTLPIAQYPTIAPPAVQISATYPGASAKTVENT
VTQVIEQQMSGLDHLLYLSSTSDDSGTATITLTFAAGTNPDIAQVQVQNKLQLATPLLPQ
AVQQLGTKVTKSSSSFLLVMAFVSEDGSMNKYDLANYVASNIQDPVSRIDGVGTVTLFGS
QYAMRIWLDANKLTNFGLTPVDVETALQAQNVQVAGGSLGGTPSVPGQVLQATITEATLL
NTPEQFGNVLLKVNQDGSRVRIKDVARIELGGENYNFDTKYNGQPTAGFGIQLATGANAL
ATAKAVRAKVAELSKYFPHGLVVQYPYDTTPFVRLSIEEVVKTLLEGIVLVFLVMYLFLQ
NLRATLIPTIAVPVVLLGTFAIMGLVGFSINTLSMFGLVLAIGLLVDDAIVVVENVERVM
QEEGLSPRDATRKAMDQITGALVGVALVLSAVFVPVAFSGGSVGAIYRQFSLTIVAAMVL
SVLVALILTPALCATILKPIEQGHQEKTTGFFGWFNRTFNKSRDKYHSGVHHVIKRSGRW
LIIYMVVIFAVGLLFVKLPKSFLPDEDQGTMFVLVQTPSGSTQETTARALKDVSDYLLNT
EKSIVESTFTVNGFSFAGRGQNAGLVFVRMKDYAQRQHSDQKVQALVGRLFMHFGSYKNA
LVYPVNPPSIPELGTAAGFDFELQDRAGVGHEKLMEARNMLLGMAAKDPTLAQVRPNGLN
DTPQFKVDIDHEKASALGVSLSAIDQTFSIAWASQYVNNFLDTDGRIKKVYLQGDAPFRM
TPEDLNAWYVRNSAGGMVPFSSFASGTWTYGSPKLERYNGISAVEIQGAAAPGKSTGQAM
TAMEALVAKLPAGVGYEWTGLSLQERQSGSQAPILYGISILVVFLCLAALYESWSIPFSV
IMVVPLGVIGALLAATLRGLENDVFFQVGLLTTVGLSAKNAILIVEFARELQQGEGMGPI
EAALEAARLRLRPILMTSLAFILGVMPLAISNGAGSASQHAIGTGVIGGMLTATFLAIFM
IPMFFVVIRAKFGGEKEDPDEALAHYNQHHPHDGQGGGSAGGSADEGSGKDGH