Protein Info for BPHYT_RS16105 in Burkholderia phytofirmans PsJN

Updated annotation (from data): ABC transporter for D-Sorbitol, permease component 1
Rationale: Specific phenotypes on D-Sorbitol.
Original annotation: mannitol ABC transporter permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 291 transmembrane" amino acids 30 to 51 (22 residues), see Phobius details amino acids 88 to 112 (25 residues), see Phobius details amino acids 123 to 146 (24 residues), see Phobius details amino acids 158 to 179 (22 residues), see Phobius details amino acids 200 to 223 (24 residues), see Phobius details amino acids 255 to 276 (22 residues), see Phobius details PF00528: BPD_transp_1" amino acids 105 to 278 (174 residues), 50.9 bits, see alignment E=7.9e-18

Best Hits

KEGG orthology group: K10229, sorbitol/mannitol transport system permease protein (inferred from 100% identity to bpy:Bphyt_3244)

MetaCyc: 66% identical to polyol ABC-type transporter permease component MtlG (Pseudomonas fluorescens)
7.5.2.M2 [EC: 7.5.2.M2]; 7.5.2.M2 [EC: 7.5.2.M2]; 7.5.2.M2 [EC: 7.5.2.M2]

Predicted SEED Role

"Various polyols ABC transporter, permease component 2" in subsystem Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.5.2.M2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SYA0 at UniProt or InterPro

Protein Sequence (291 amino acids)

>BPHYT_RS16105 ABC transporter for D-Sorbitol, permease component 1 (Burkholderia phytofirmans PsJN)
MSQVASTPVSNPTAMTVPAKSPFAAIRRGIPGVIAWLVALLLFFPIFWMTITAFKTEQQA
YASSLFFIPTLDSFREVFARSNYFSFAWNSILISAGVTILCLILAVPAAYAMAFFPTRRT
QKVLLWMLSTKMMPSVGVLVPIYLLWKNSGLLDSVSGLVIVYTLINLPIAVWMSFTYFAE
IPRDILEAGRIDGAATWQEIVYLLMPMSLPGLASTALLLVILSWNEAFWSINLSSSNAAP
LTVFIASYSSPEGLFWAKLSAASLLAVAPILIVGWLSQKQLVRGLTFGAVK