Protein Info for BPHYT_RS15935 in Burkholderia phytofirmans PsJN

Annotation: glycerol-3-phosphate acyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 214 transmembrane" amino acids 6 to 28 (23 residues), see Phobius details amino acids 52 to 72 (21 residues), see Phobius details amino acids 84 to 102 (19 residues), see Phobius details amino acids 114 to 138 (25 residues), see Phobius details amino acids 158 to 179 (22 residues), see Phobius details TIGR00023: acyl-phosphate glycerol 3-phosphate acyltransferase" amino acids 3 to 197 (195 residues), 191.3 bits, see alignment E=7.8e-61 PF02660: G3P_acyltransf" amino acids 9 to 188 (180 residues), 182.9 bits, see alignment E=2.6e-58

Best Hits

Swiss-Prot: 100% identical to PLSY_PARPJ: Glycerol-3-phosphate acyltransferase (plsY) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K08591, glycerol-3-phosphate acyltransferase PlsY [EC: 2.3.1.15] (inferred from 100% identity to bpy:Bphyt_3209)

Predicted SEED Role

"Acyl-phosphate:glycerol-3-phosphate O-acyltransferase PlsY" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.15

Use Curated BLAST to search for 2.3.1.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SY66 at UniProt or InterPro

Protein Sequence (214 amino acids)

>BPHYT_RS15935 glycerol-3-phosphate acyltransferase (Burkholderia phytofirmans PsJN)
MQNLIVAVVAYLIGSVSFAVIVSAAMGLDDPRSYGSGNPGATNVLRSGNKKAAILTLIGD
AFKGWLPVWFVVHYGARYGLDETSVAIASVAVFLGHLYPVFFRFKGGKGVATAAGVLLAI
NPILGVATLLTWLIVAFFTRYSSLAALAAAVFAPIFDGFLFGPHVIALAIVVMSSLLVWR
HRGNIAKLMRGQESRIGDKKKADAAAKPAGGSDV