Protein Info for BPHYT_RS15890 in Burkholderia phytofirmans PsJN

Annotation: 3-hydroxy-2-methylbutyryl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00106: adh_short
  • amino acids 7 to 196 (190 residues), 155.7 bits, E=1.6e-49
• PF08659: KR
  • amino acids 8 to 163 (156 residues), 28.5 bits, E=2.1e-10
• PF13561: adh_short_C2
  • amino acids 14 to 244 (231 residues), 136.5 bits, E=1.7e-43

Best Hits

Swiss-Prot: 58% identical to HCD2_DROME: 3-hydroxyacyl-CoA dehydrogenase type-2 (scu) from Drosophila melanogaster

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_3200)

MetaCyc: 62% identical to 3-hydroxy-2-methylbutyryl-CoA dehydrogenase subunit (Pseudomonas putida)
3-hydroxy-2-methylbutyryl-CoA dehydrogenase. [EC: 1.1.1.178]

Predicted SEED Role

"3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100)" in subsystem Fatty Acid Biosynthesis FASII or mycolic acid synthesis (EC 1.1.1.100)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (50/53 steps found)
• palmitate biosynthesis III (28/29 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• anteiso-branched-chain fatty acid biosynthesis (30/34 steps found)
• even iso-branched-chain fatty acid biosynthesis (30/34 steps found)
• odd iso-branched-chain fatty acid biosynthesis (30/34 steps found)
• tetradecanoate biosynthesis (mitochondria) (23/25 steps found)
• superpathway of unsaturated fatty acids biosynthesis (E. coli) (19/20 steps found)
• octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) (12/12 steps found)
• oleate biosynthesis IV (anaerobic) (13/14 steps found)
• palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (9/9 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (14/16 steps found)
• biotin biosynthesis I (13/15 steps found)
• cis-vaccenate biosynthesis (5/5 steps found)
• fatty acid elongation -- saturated (5/5 steps found)
• gondoate biosynthesis (anaerobic) (4/4 steps found)
• 8-amino-7-oxononanoate biosynthesis I (9/11 steps found)
• (5Z)-dodecenoate biosynthesis I (5/6 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• 8-amino-7-oxononanoate biosynthesis IV (4/5 steps found)
• (5Z)-dodecenoate biosynthesis II (4/6 steps found)
• L-isoleucine degradation I (4/6 steps found)
• propanoate fermentation to 2-methylbutanoate (4/6 steps found)
• stearate biosynthesis IV (4/6 steps found)
• 2-methyl-branched fatty acid β-oxidation (9/14 steps found)
• petroselinate biosynthesis (2/6 steps found)
• 2-allylmalonyl-CoA biosynthesis (2/8 steps found)
• streptorubin B biosynthesis (20/34 steps found)
• mycolate biosynthesis (25/205 steps found)
• superpathway of mycolate biosynthesis (26/239 steps found)

KEGG Metabolic Maps

• Biosynthesis of unsaturated fatty acids
• Fatty acid biosynthesis
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.100

Use Curated BLAST to search for 1.1.1.100 or 1.1.1.178

Sequence Analysis Tools

See B2T6M5 at UniProt or InterPro

Protein Sequence (252 amino acids)

>BPHYT_RS15890 3-hydroxy-2-methylbutyryl-CoA dehydrogenase (Burkholderia phytofirmans PsJN)
MEIRDNVFLITGGASGLGAATARLFVENGGKVVLADLNVDAGEALAKELGGVFVRCDVSR
EDDATQAVEAATKLGTLRGLVNCAGVAPAVKTVGKDGPHPLDAFARTISINLIGTFNMVR
LAAAAMSKNEPNANGERGVIINTASVAAFDGQIGQAAYAASKAGVAGMTLPIARDLSRNA
IRVMTIAPGIFETPMLLGMPQEVQDALGAMVPFPPRLGKPVEYAMLAKQIFDNPMLNGEV
IRLDGAIRMQPK