Protein Info for BPHYT_RS15735 in Burkholderia phytofirmans PsJN

Annotation: pseudouridine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00849: PseudoU_synth_2
  • amino acids 2 to 148 (147 residues), 71.1 bits, E=6.1e-24
• TIGR00093: pseudouridine synthase
  • amino acids 7 to 180 (174 residues), 158.6 bits, E=5.7e-51

Best Hits

Swiss-Prot: 61% identical to RLUE_PSEAE: Ribosomal large subunit pseudouridine synthase E (rluE) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K06181, ribosomal large subunit pseudouridine synthase E [EC: 5.4.99.12] (inferred from 100% identity to bpy:Bphyt_3170)

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase E (EC 4.2.1.70)" (EC 4.2.1.70)

MetaCyc Pathways

• pseudouridine degradation (1/2 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

See B2T6J8 at UniProt or InterPro

Protein Sequence (189 amino acids)

>BPHYT_RS15735 pseudouridine synthase (Burkholderia phytofirmans PsJN)
MHLLALNKPFGTICQFSPHETRASLADWVKVPDVYPAGRLDSDSEGLLLLTDDGALQARI
AEPRHKLVKRYWAQVEGAVDAATLKKLARGVDLGDYVTRPCRAEYVEPTDALWTRTPPIR
FRAAIPTTWIELSITEGKNRQVRRMTAAVGFPTLRLVRVGIGALDIFSLGLQPGESVELS
PKAPWEGIA