Protein Info for BPHYT_RS15675 in Burkholderia phytofirmans PsJN

Annotation: riboflavin kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 331 PF06574: FAD_syn" amino acids 11 to 166 (156 residues), 184.7 bits, see alignment E=1.7e-58 TIGR00083: riboflavin biosynthesis protein RibF" amino acids 17 to 307 (291 residues), 282.5 bits, see alignment E=4.1e-88 TIGR00125: cytidyltransferase-like domain" amino acids 17 to 75 (59 residues), 27.9 bits, see alignment E=2.1e-10 PF01467: CTP_transf_like" amino acids 18 to 170 (153 residues), 28.3 bits, see alignment E=2.7e-10 PF01687: Flavokinase" amino acids 184 to 307 (124 residues), 126.9 bits, see alignment E=8.3e-41

Best Hits

Swiss-Prot: 48% identical to RIBF_PSEFL: Bifunctional riboflavin kinase/FMN adenylyltransferase (ribF) from Pseudomonas fluorescens

KEGG orthology group: K11753, riboflavin kinase / FMN adenylyltransferase [EC: 2.7.1.26 2.7.7.2] (inferred from 100% identity to bpy:Bphyt_3158)

MetaCyc: 45% identical to bifunctional riboflavin kinase / FMN adenylyltransferase (Escherichia coli K-12 substr. MG1655)
FAD synthetase. [EC: 2.7.7.2]; Riboflavin kinase. [EC: 2.7.7.2, 2.7.1.26]

Predicted SEED Role

"Riboflavin kinase (EC 2.7.1.26) / FMN adenylyltransferase (EC 2.7.7.2)" in subsystem Riboflavin, FMN and FAD metabolism (EC 2.7.1.26, EC 2.7.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.26 or 2.7.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T6I6 at UniProt or InterPro

Protein Sequence (331 amino acids)

>BPHYT_RS15675 riboflavin kinase (Burkholderia phytofirmans PsJN)
MRVFRGLPNAESRAPCALTIGNFDGVHRGHQALLAHVRAAADARGLPVCVMTFEPHPREF
FNPAGAPPRIAMLRDKLEALRTNGVDRVVVEHFNHTFASQSPDTFVERIIVNGLHARWVM
IGDDFRYGAKRAGDFDSLKAAGQQYGFEVEQMATVADPSGARISSSGVRAALVAGDLDSA
RAALGRDYLISGHVVHGMKLGRDLGFPTLNLAIAHKRPALSGIFVVRVHGVADEPLPGVA
SLGLRPTVDDSGRVLLEVHLLDWHGDAYGKLVRVEFLKKLRDEEKYVDLETLTAAIARDV
ANARAWFAAVGAGTPGSRSTGFATSATDRIR