Protein Info for BPHYT_RS15560 in Burkholderia phytofirmans PsJN

Annotation: pseudouridine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00849: PseudoU_synth_2
  • amino acids 64 to 195 (132 residues), 65.8 bits, E=2.8e-22
• TIGR00093: pseudouridine synthase
  • amino acids 68 to 226 (159 residues), 127.4 bits, E=2.2e-41

Best Hits

KEGG orthology group: K06183, ribosomal small subunit pseudouridine synthase A [EC: 5.4.99.12] (inferred from 100% identity to bpy:Bphyt_3135)

Predicted SEED Role

"Ribosomal small subunit pseudouridine synthase A (EC 4.2.1.70)" (EC 4.2.1.70)

MetaCyc Pathways

• pseudouridine degradation (1/2 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

See B2T6G3 at UniProt or InterPro

Protein Sequence (236 amino acids)

>BPHYT_RS15560 pseudouridine synthase (Burkholderia phytofirmans PsJN)
MNLESILFTQGFGSRRQCRALIADARVSIGGVACTDADADFPTGDTAFFFSVDGVDWPYR
EHAYLLLNKPAGYECSRDPQHHLSVFSLLPPQFAERGVQCVGRLDQDTTGLLLLSDDGKF
VHLFTSPKRKVPKVYIATTRHPLDDAQLSALRDGVLLHGEPKPIAAVDAAARGANTLALT
VMEGKYHQVKRMIAAAGNRCEALHRERIGGLALPETLAPGAWQWLDETDLGSLRSG