Protein Info for BPHYT_RS15325 in Burkholderia phytofirmans PsJN

Annotation: ribonuclease PH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 246 TIGR01966: ribonuclease PH" amino acids 7 to 241 (235 residues), 388.8 bits, see alignment E=3.7e-121 PF01138: RNase_PH" amino acids 15 to 145 (131 residues), 104.5 bits, see alignment E=5.8e-34 PF03725: RNase_PH_C" amino acids 162 to 228 (67 residues), 56.8 bits, see alignment E=1.8e-19

Best Hits

Swiss-Prot: 100% identical to RNPH_PARPJ: Ribonuclease PH (rph) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K00989, ribonuclease PH [EC: 2.7.7.56] (inferred from 100% identity to bpy:Bphyt_3087)

Predicted SEED Role

"Ribonuclease PH (EC 2.7.7.56)" in subsystem Heat shock dnaK gene cluster extended or tRNA processing (EC 2.7.7.56)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.56

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T6B5 at UniProt or InterPro

Protein Sequence (246 amino acids)

>BPHYT_RS15325 ribonuclease PH (Burkholderia phytofirmans PsJN)
MTNDTQRPSGRQANQLRDVRITRHYTKHAEGSVLVEFGDTKVICTASIAESVPSFLRDRG
QGWLTAEYGMLPRATHTRSDREAARGKQTGRTQEIQRLIGRALRSVFDLEKLGARTLHID
CDVIQADGGTRTASITGAFVAAHDAVAKLLATGRIESSPITDYVAAISVGVYDGLPVLDL
DYDEDSQCDTDMNVVMTGAGGFVEIQGTAEGVAFSRDEMNALLDLASDGINTLIAKQKAA
LEQKSE